Isaac Melamed*, Lacey Robinson and Melinda Heffron
IMMUNOe International Research Centers, Centennial, CO, USA
Received: 10 April, 2017; Accepted: 03 May, 2017; Published: 04 May, 2017
Isaac Melamed, ImmunoE Health Centers, 6801 South Yosemite Street, Centennial, USA, Tel: 303-224-4678; 720-273-2863; Fax: 303-224-4699; E-mail:
Melamed I, Robinson L, Heffron M (2017) The Benefit of Montelukast in Atopic Dermatitis Induced by Food Allergies. Glob J Allergy 3(1): 013-018. DOI: 10.17352/2455-8141.000018
© 2017 Patella V, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background: Cysteinyl leukotriene levels are elevated in patients with atopic dermatitis, which can lead to eosinophilic infiltration of the gastrointestinal tract.
Objective: We examined the role that montelukast (a leukotriene receptor antagonist) might play in improving symptoms of atopic dermatitis induced by food allergies.
Methods: We conducted a randomized, double-blind, placebo-controlled, parallel group study in 20 children, aged 1 to 8 years, with 4 study visits every 3 weeks for 9 weeks. Primary inclusion criteria consisted of: 1) positive reactivity to food (indicated by skin or RAST test); 2) 10–25 % body area affected with atopic dermatitis; and 3) gastrointestinal symptoms. Liquid cetirizine and 1% hydrocortisone cream were both given as rescue medications for atopic dermatitis flare-ups. Pruritis and atopic dermatitis flare-up scores were used to collect clinical data. Laboratory values for nerve growth factor were evaluated pre- and post-treatment.
Results: Our main endpoints were the effects of montelukast on the clinical presentation of atopic dermatitis. When comparing the treatment group to placebo, we noted a significant reduction in the pruritis score (p=0.002); a trend toward a reduction in the use of rescue medication (cetirizine: p=0.056; hydrocortisone cream: p=0.056); and a reduction in the level of nerve growth factor; mean values: placebo=3.06 to montelukast=2.59.
Conclusion: The inflammatory pathway triggered by food allergies that may lead to atopic dermatitis can be modulated with montelukast. Furthermore, nerve growth factor may play a role in the pathogenesis of atopic dermatitis and montelukast may modify this pathway
Atopic dermatitis (AD) is a chronic, relapsing, inflammatory skin disorder . The inflammatory process seen in AD is characterized by a mixture of immunological and pharmacological abnormalities that can be detected in the serum and at the cellular level. Included in the immune histology of AD skin are infiltrates of activated T-cells, dendrite presenting cells, eosinophils, and mast cells .
Commencement of the immune cascade can result from multiple activators/activations, including allergens (food or environmental), infectious pathogens, and numerous other distinctive triggers (eg, stress or anxiety) . The cascade involves a series of reactions that culminate in different biologic responses, including entry into the cell cycle or cell death. While programmed cell death, or apoptosis, can be triggered under a variety of circumstances, the mechanism by which cells are directed to cell cycle progression as opposed to apoptosis is not well understood . In recent years, increasing evidence has suggested that regulatory T-cells are involved in various skin diseases and that they play a pivotal role in the etiology of AD . These roles are complex and may involve various mechanisms, showing that the immune response leading to inflammation due to a failure of apoptosis is a crucial factor [6,7].
Cysteinyl leukotrienes (Cys-LTs) are the products of arachidonic acid metabolism and are released from various cells, including mast cells, basophils, and eosinophils; they have long been known to be potent mediators of allergic inflammation. The secretion of these products (eicosanoids) and the occupation of the Cys-LT receptors have been correlated with the pathophysiology of both asthma and allergic rhinitis in numerous studies. It has recently been suggested that Cys-LTs may also play a role in food allergies and their subsequent immune response [8-12].
Recent studies have shown that some food allergens can increase the levels of Cys-LTs [8-12]. These studies suggested that leukotriene receptor antagonists could potentially interfere with the inflammatory cascade induced by food allergens. A recent study by Adamek-Guzik et al found that the levels of Cys-LTs in patients with AD were significantly higher during skin flare-up compared to the remission phase . It also noted that the clinical severity of skin lesions correlated with increased levels of Cys-LTs.
Other studies concur with the use of leukotriene receptor antagonists for AD patients [7,12]. These studies show a correlation between the occurrence of AD in patients with known food intolerances. In the presence of food allergens, increased Cys-LT production by peripheral leukocytes (specifically eosinophils) was observed in the majority of patients. Eosinophilic involvement in the pathogenesis of AD has previously been established. The immune response in AD causes an increase in the amount of Cys-LTs brought about by food intolerance. These studies also suggest the use of leukotriene receptor antagonists for reduction in the severity of AD for patients with food allergies.
The importance of eosinophilic involvement in the inflammatory response is also found to occur in gastrointestinal (GI) disorders [10,11]. The infiltration of eosinophils into the GI tract is seen in eosinophilic gastroenteritis (EG), which causes severe tissue damage to the gut. Food allergies are a triggering factor in the allergic response, thereby causing the accumulation of eosinophils in the tissues of the GI tract. EG and AD are strongly associated with known food allergies in about 70% of the cases.
In vitro studies examined the effect of leukotriene B4 (LTB4) antagonists on eosinophilic infiltration in the gut and skin , BALB/c mice received an oral challenge to induce eosinophilic infiltration into the gut and skin. Peak infiltration occurred at 6 hours (gut) and 12 hours (skin) after the challenge. If an intraperitoneal dosage of a LTB4 antagonist was administered before the oral challenge, a significant inhibition of eosinophilic infiltration was noted. A reduction of 53.3% in the skin and 73.3% in the gut was measured. Although these results were not conducted in humans, they point to the advisability of using a leukotriene inhibitor to prevent eosinophilic infiltration in the skin and gut in cases of known food allergies.
Considerable evidence suggests a crosstalk between the nervous and immune systems. Previously, we investigated the role of nerve growth factor (NGF) in this crosstalk and showed an active role for NGF in B-cell cytoskeletal signaling [14-19]. It has been suggested that NGF plays a role in the inflammatory process and in tissue repair . Both skin and lung fibroblasts produce NGF and express tyrosine kinase receptor under basal conditions. In cutaneous inflammatory responses, the expression of NGF receptor (TrkA) is reduced following an acute inflammatory stimulus and also in association with chronic inflammatory dermatitis [21,22], suggesting that NGF plays an active role in the pathogenesis of AD .
Leukotriene receptor antagonists are specific in blocking the Cys-LT receptor, thereby preventing the immune cascade response. Montelukast, the active ingredient in the product Singulair®, has been shown to be a selective and potent leukotriene receptor antagonist and has been studied extensively for its use in the reduction or prevention of airway inflammation and allergic rhinitis. As leukotriene B4 (LTB4) antagonists may reduce eosinophilic infiltration into the gut and skin, we formed a hypothesis regarding the role of food allergens in the induction of eosinophilic activation and their contribution to dermatological symptoms.
Our hypothesis for this research study was that NGF may play a role in the inflammatory process and that montelukast would possibly:
1. Downregulate the inflammatory process caused by food allergy triggers
2. Downregulate NGF
3. Improve the symptoms of AD.
In this randomized, double-blind, placebo-controlled, parallel-group trial, participants diagnosed with AD and food allergies aged 1-8 years were enrolled. The study was conducted at 2 outpatient clinics in the Denver, Colorado, metropolitan area between February 2008 and March 2009. Individual subject participation lasted for approximately 9 weeks. The study was IRB-approved with informed consent and registered with ClinicalTrials.gov. (Quorum Review IRB # 23389/1; ClinicalTrials.gov Identifier: NCT00557284).
Thirty-three subjects were screened for the trial; 20 subjects met all inclusion and exclusion criteria. Nine subjects were randomized to receive montelukast and 11 subjects were randomized to receive placebo. Eleven males and 9 females were enrolled. The mean age of participants was 5.4 years.
All entry criteria were assessed at the baseline visit. Using age at the time of screening (1-8 years inclusive), participants with mild to moderate AD affecting at least 5% of the body surface with a total severity score, rated by the study doctor, of 2 for any of the 4 signs, were enrolled. GI symptoms were rated by the Gastrointestinal Symptom Rating Scale (GSRS) and a total score of 2 was necessary for inclusion. If participants had previously tested positive for food allergens by skin or radioallergosorbent test (RAST) test, the previous results were used. If participants had not been tested for food allergens, then the necessary tests (RAST and skin tests) were performed to determine eligibility. Subjects were seen in clinic by a study doctor a total of 4 times, once every 21 to 23 days, over a 9-week study period. Standard of care in the clinic is to avoid foods that may lead to anaphylaxis as determined by RAST or skin testing.
Skin evaluations were performed by the study doctor at study visits using Investigators’ Global Assessment (IGA) rating, severity scores, and percent of body area affected. Blood was taken at visit 1 and 4 and urine samples were taken at each visit. Caregivers filled out weekly assessments of disease control, pruritus evaluation, and AD flare-up cards. GI symptoms were evaluated weekly by the caregiver using the GSRS scale. Visit 1 scores were considered baseline. Non-serious adverse events (NSAES) and serious adverse events (SAES) were recorded, monitored, and reported according to protocol.
The study consisted of 2 treatments arms: those who received montelukast and those who received placebo. All study drugs, montelukast or placebo, were dosed orally one time per day. Subjects aged 2 to 5 years of age received 4-mg chewable tablets and subjects 6 to 8 years of age received 5-mg chewable tablets. Second line therapy provided for treatment of AD flares when symptoms became unacceptable to the participants consisted of 1% hydrocortisone cream applied to affected areas and cetirizine HCl (Zyrtec®) liquid. Subjects 1 to 4 years of age received ½ teaspoon of cetrizine once daily as needed and subjects 5 to 8 years of age received 1 teaspoon once daily as needed.
Demographic information, medical history, and medication history were collected at the initial visit. All concomitant medications were recorded by the caregiver in the diary card and reviewed at study visits for the duration of the trial. Several validated scores were used for evaluation during the trial. The IGA score is a 6-point measure of disease severity based on the clinicians overall assessment of skin lesions: 0=clear, 1=almost clear, 2=mild, 3=moderate, 4=severe, and 5=very severe. Subjects did not need to have a specific IGA score to qualify for the trial. The IGA was assessed at each of the 4 study visits. The score recorded at visit 1 was considered the baseline score; caregiver’s assessment score was the evaluation made by the caregiver on the perceived level of disease control over the previous 7 days. Level of control was ranked on a 4-point scale ranging from complete control (0) to uncontrolled disease (3). The caregiver recorded this score on a weekly basis in the diary card. Pruritus (“itch”) assessments were recorded by the caregiver for the previous 24 hours using a 4-point scale, ranging from none (0) to severe (3). This assessment was recorded daily in the diary card. AD flare-ups were documented. A flare-up is defined as a worsening of disease that is unacceptable to the participants and leads to second-line topical steroid use and/or liquid antihistamine use. The caregivers documented flare-ups by recording the use of second-line therapy in the diary card. GI symptoms were recorded weekly on a GSRS-validated scale adjusted for pediatric patients.
Urine samples were obtained at all study day 4 visits for all subjects. The urine sample at visit 1 was considered the baseline. Urine samples were analyzed for levels of Cys-LTs.
Serum samples were collected at the first study visit and the last. A chemistry panel, including albumin, alkaline phosphatase, serum glutamic pyruvic transaminase (SGPT), serum glutamic oxaloacetic transaminase (SGOT), and blood urea nitrogen (BUN) as well as a complete blood count (CBC), were performed as a safety precaution. No safety concerns were identified by these tests. Serum markers were also obtained for immunoglobulin E (IgE), interleukin-5 (IL-5), interleukin-13 (IL-13), tumor necrosis factor-alpha (TNF-α), and NGF. Chemistry and IgE levels were run by Quest Diagnostics. IL-5, IL-13, TNF-α, NGF, and urine LTE-4 were measured using ELISA at ELISATech.
Treatment effects were analyzed using repeated measures, including ANOVA methods, which take into account correlation within subjects. Individual contrasts were calculated to compare treatment means of interest. All p-values were two-sided; all analyses were performed using SAS (SAS Institute, Inc., version 9.1).
Primary endpoint evaluations – Skin evaluations
The primary endpoints for skin evaluations were IGA, caregivers’ assessment of disease control (PADC), pruritis assessment, and AD flare-up. Pruritis scores and PADC were measured on a daily basis. AD flare-up was documented by tracking the number of times rescue medicines (liquid cetirizine and/or 1% hydrocortisone cream) were required to control symptoms.
The overall skin level of discomfort using PADC, pruritis, and AD flare-up was greatly improved in subjects using montelukast compared to those using placebo (Tables 1-3). Figure 1 depicts the average weekly pruritus scores by treatment group. Study visits compared to baseline followed a downward trend in the montelukast group, with the difference being most significant by the last visit. When comparing pruritis baseline scores to last study visit scores, the difference between placebo and montelukast was significant (p=0.002). There was a difference in use of cetirizine (p=0.056) and hydrocortisone cream (p=0.054) between the 2 groups, but it was not significant. A similar trend was also noticed for PADC (p=0.248).
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