Hossein Saghi1, Abbas Bahador2, Ferdoes Amiri Dastjerdi1, Hassan Asadolahi1, Mehdi Neyshaboori1, Esmaeili Mohammad 3, Meskini Maryam3 and Davoud Esmaeili3*
1AJA University of Medical Sciences, Tehran, IR Iran
2Department of Medical Microbiology, Tehran University of Medical Sciences, Iran
3Microbiology Department Baqiyatallah University Medical of Sciences, Iran
Received: 21 September, 2015; Accepted: 26 October, 2015; Published: 28 October, 2015
Davoud Esmaeili, Department of Microbiology, Baqiyatallah University of Medical Sciences, Tehran, IR Iran. Tel: +989124395884; Fax: +98-2177601533; E-mail:
Saghi H, Bahador A, Dastjerdi FA, Asadolahi H, Neyshaboori M, et al. (2015) Antibacterial Effects of Herbal Compounds against Acinetobacter baumannii Isolated from Hospital of Tehran, Iran. Glob J Infect Dis Clin Res 1(1): 018-020.DOI: 10.17352/2455-5363.000005
© 2015 Saghi H, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Acinetobacter baumannii; Antibacterial Effects; Multidrug-resistant; Satureja essence; MIC
Increasing isolation of multidrug-resistant Acinetobacter baumannii (MDR) has been reported in worldwide and it is recently one of the most difficult nosocomial acquired gram-negative pathogens to control and treatment. These bacteria are one of ESBL producing that great potential have for the rapid development of antibiotic resistance. Use of medicinal herbaceous drugs recommended for treatment since ancient periods. Humans have been used and realize their beneficial effects. With the increase in population and urban growth, reduced use of synthetic drugs, many of these of medicinal herbs have been replaced. Because of Satureja, Origanum vulgare and Thyme essence has antimicrobial properties so can be used against infections caused by MDR Acinetobacter baumannii. In this study the antimicrobial effects of Satureja, Origanum vulgare and Thyme essence on drug-resistant strains of Acinetobacter baumannii were investigated using antimicrobial analysis with CLSI 2013 and Kirby Bauer method. The agar dilution method results revealed that Satureja essence compare with Origanum vulgare and Thyme had strong inhibitory effects against Multidrug-resistant strains of A. baumannii. Because of proper effects of plant essential oils, with a broader range of studies can be used as a complementary therapy.
Acinetobacter baumannii is a gram-negative bacteria and important opportunistic pathogen, causes a variety of nosocomial infections especially in Intensive Care Units (ICU's) [1,2]. These infections include bacteremia, surgical-site infections, secondary meningitis, urinary tract infections and ventilator associated pneumonia. These organisms have been implicated in a diverse range of infections such as respiratory tract, blood stream, skin and soft tissue. The rapid emergence and global dissemination of A. baumannii as a major nosocomial pathogen is remarkable and demonstrates its successful adaptation to the 21st century hospital environments . Use of medicinal herbaceous drugs recommended for treatment since ancient periods. Humans have been used and realize their beneficial effects. With the increase in population and urban growth, and reduced use of synthetic drugs, many of these of medicinal herbs have been replaced . The usage problems such as the increasing resistance of microorganisms and reduce the impact of the continuing application is reported.
Materials and Methods
Extraction, isolation and identification of the oils
Aerial parts of Satureja and Thyme were collected from Barij Essence research farm in May 2010. Plant material and extraction procedure flowering aerial parts of O. vulgare was collected from Khoramabad, Lorestan, Iran, in June 2010. Essential oils by steam distillation of the aerial parts of the plant were prepared aerial. Oil after drying with sodium sulfate at 4°C until the GC (gas chromatography) injection system was kept. Analysis and identification of constituent composition of the oils was performed by Barij Essence Company. The fresh flowering aerial parts of Satureja, Origanum vulgare and Thyme were subjected to hydro distillation and a yield of 0.27% (v/w) was obtained. The GC /MS (gas chromatography–mass spectrometer) results indicated that these oils have many antibacterial compounds. The GC apparatus was Agilent technology (HP) 6890 system, capillary column of HP-5MS (60m×0.25mm, film thickness 0.25_m). The oven temperature program was initiated at 40°C, held for 1 min then raised up to 230°C at a rate of 3°C/min held for 10min. Helium was used as the carrier gas at a flow rate 1.0 ml/min.
Bacterial strain and culture conditions
The number of seventy five Acinetobacter baumannii strains was isolated and identified using standard microbiological methods. Susceptibility testing of antibiotics neomycin, gentamicin, amikacin, kanamycin, imipenem and oxacillin were performed. The resistant strains were used to investigate in order to antimicrobial effect of Satureja, Origanum vulgare and Thyme essence. To evaluate the antimicrobial effects Satureja, Origanum vulgare and Thyme essential oil diffusion method (disk diffusion) were used. We use dimethyl sulfoxide (DMSO) for dissolve essential oil. Culture carried out with sterile swab and micro tube suspension was cultured for 24 h and then inoculated onto Mueller Hinton agar. Blank discs with a diameter of 6 mm and containing 30 µl of the concentration of essential oils were placed on Muller Hinton agar medium. After 24 h incubation at 37°C, zones of growth inhibition were measured. Each concentration was repeated 3 times for each of the bacteria and the average was documented. Disks containing 30 µl of dimethyl sulfoxide were used as a negative control. To determine the minimum inhibitory concentration (MIC) for the Satureja, Origanum vulgare and Thyme essential oil to 100 µl (100% concentration) was dissolved in 900 µl DMSO. Then the 1, 2, 3, 4, 5, 6, 7, 8,9,10 µg of this suspension was added to 1 ml Muller Hinton broth. After mixing, 1 ml of bacterial suspension (1.5×108 cfu / ml) was added to the medium after incubation at 37°C for 24 h and the results were evaluated. Acinetobacter baumannii ATCC 19606 was employed in this study as a model reference strain.
Results and Discussion
Antibiotic susceptibility test results the number of seventy five Acinetobacter baumannii strains are as follows. Oxacillin (100%), Amikacin (75%), Kanamycin (68%), Gentamicin (60%), Imipenem (60%) and (89%) were resistant Neomycin. Inhibitory effects of Satureja on drug-resistant strains were found. Results MIC (µg/ml) Satureja, Origanum vulgare and Thyme essential oils and Zone of growth inhibition (mm) pathogen strain Acinetobacter baumannii (Table 1) (Figure 1). The major components of Satureja essence were carvacrol (90.88%), ρ-cymene (3.11%), γ - trepanned (1.24%), linalool (0.91%), Origanum vulgare are Pulegone (68.59%), Piperitenone Oxide and Piperitone (7.8%) and the Thyme are thymol (28.8%) and carvacrol (23.46%).
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