Abstract

    Open Access Research Article Article ID: ACG-5-163

    Long non-coding RNA HOXA11-AS facilitates cell migration and invasion by regulating microRNA-148a/WNT1 axis in gastric cancer

    Tao Guo, Xiao Yuan, De-Feng Liu, Shi-Hao Peng and A-Man Xu*

    Recently, long non-coding RNAs (lncRNAs) have emerged as crucial players in the initiation and progression of multiple cancers including gastric cancer (GC). LncRNA HOXA11 antisense RNA (HOXA11- AS) has been found to be an oncogenic factor in GC. However, the molecular mechanisms responsible for these oncogenic properties have not been thoroughly elucidated. In the present study, the abundances of HOXA11-AS, miR-148a and WNT1 in GC tissues and cell lines were examined by qRT-PCR. Kaplan- Meier survival analysis was conducted to explore the relationship between HOXA11-AS expression and GC patient prognosis. Transwell assay was performed to evaluate cell migratory and invasive capacities. Bioinformatics, dual-luciferase reporter, RNA immunoprecipitation (RIP), and RNA-pull down assays were employed to analyze the interaction between miR-148a and HOXA11-AS or WNT1.

    Keywords:

    Published on: Jul 23, 2019 Pages: 19-26

    Full Text PDF Full Text HTML DOI: 10.17352/2455-2283.000063
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