Background: The possibility of culturing heart cells in order to regenerate damaged tissue is a challenging problem. Recent observations have demonstrated the possibility of isolating and expanding resident cardiac stem cells, which could favor regeneration and functional improvement of the myocardial tissue.
Aims: To investigate two different culturing media: one promoting c-kit cells’ growth and the other promoting differentiation in cardiac muscle cells.
Methods: We obtained primary cultures from left ventricle myocardial tissues of 10 human hearts of patients with end-stage heart failure who received heart transplantation. Cells were first cultured in a medium containing high serum and low calcium/magnesium (Ca2+ /Mg2+) to promote cell growth (medium A). Than they were cultured in another medium that contained lower serum concentration and a variety of different factors in order to induce cell differentiation (medium B). The presence of c-kit, specific for stem cells, α-sarcomeric actin (SA), specific for skeletal and cardiac muscle cells, and α-smooth muscle actin (SMA), specific for smooth muscle cells was studied by immune-cytochemical analysis.
Results: A high percentage of c-kit+, SMA-, SA- cells was observed in medium A; in medium B with lower serum and higher Ca2+/Mg2+ concentrations cells became c-kit-, AML+, SA+. In medium A, 78% of the cells were positive for c-kit. After culturing the same cell populations in medium B with lower serum and higher Ca2+ /Mg2+ concentrations, the percentage of c-kit positive cells decreased to 21% while the cells positive for SMA and for SA increased respectively from 28 to 82% and from 0 to 59%.
Conclusions: Our results confirm the presence of a high percentage of c-kit positive cells in failing human myocardium and, for the first time, suggest a key role of calcium/magnesium concentration in promoting both c-kit cells’ growth and their differentiation in human cardiac muscle.
Published on: Mar 7, 2015 Pages: 5-12
Columbia University, USA
Journal of Novel Physiotherapy and Physical Rehabilitation
Prof. Pierre Guertin
Laval University, Canada
International Journal of Pharmaceutical Sciences and Developmental Research
Malgorzata Gabriela WASNIEWSKA
University of Messina, Italy
International Journal of Clinical Endocrinology and Metabolism
Cardiometabolic Research Institute, USA
Journal of Cardiovascular Medicine and Cardiology
National Center for Toxicological Research (NCTR)/FDA, USA
Global Journal of Anesthesiology