Background: Inflammation and oxidative stress are two pathophysiological mechanisms that link obesity, type 2 diabetes mellitus (T2DM) and diabesity. However how levels of inflammatory and oxidative stress markers differ between patients with obesity, T2DM and diabesity has not been completely elucidated.
Objectives: This study aimed to investigate the interactions between emerging biomarkers of oxidative stress and inflammation and the differences in biomarker levels between T2DM, obesity and diabesity in a clinical setting.
Methods: A total of 270 patients attending a diabetes health screening clinic (57 T2DM; 37 obese; 44 diabesity, and 132 age, gender, and weight-matched controls) participated in the study. All patients were selected on clinical grounds. Differences in the level of biomarkers of oxidative stress (erythrocyte GSH/GSSG, 8-hydroxy-2-deoxy-guanosine (8-OHdG) and urinary 8-iso-prostaglandin F2α), inflammation (CRP, IL-6, IL-10 and IL1β), and coagulation (C5a, D-dimer) were determined.
Results: Both inflammatory markers and oxidative stress differed significantly between the three clinical groups. GSH revealed a significant difference between the T2DM (1456.6 µM/mL ± 737.5, p<0.03) and obese groups (1890.7 µM/mL ± 823.3). 8-OHdG increased significantly in the obese group (185.5ng/mL ± 162.4, p<0.03) compared to the control group (146.8ng/mL ± 127.5). Similarly 8-OHdG was significantly higher in the obesity group (185.5ng/mL ± 162.4) compared to the T2DM group (119.2ng/mL ± 92.9, p<0.03). A significant increase was also found for 8-iso-PGF2α in the diabesity group (2.3 ± 15.0 ng/mL) compared to the control group (1.0 ± 1.9 ng/mL, p<0.03) and the T2DM group (1.1±2.5ng/mL, p<0.05). 8-iso-PGF2α in the diabesity group (2.3±15.0 ng/mL) was significantly higher than the obese group (1.1ng/mL ±1.8, p<0.03). A significant decrease occurred in IGF-1 levels for diabesity (144.6 ± 285.7 ng/mL) compared to the control (302.8 ± 547.2 ng/mL, p <0.03) and the T2DM groups (225.1ng/mL ± 417, p<0.05). A statistically significant increase in the inflammatory marker ratio IL-6/IL-10 between the control group (0.41 ± 0.7) and the obesity group (0.71 ± 1.94, p<0.03) was also observed.
Conclusion: The results obtained indicate that 8-iso-PGF2α, 8-OHdG as markers of oxidative stress and IGF-1, IL-6/IL-10 are associated with diabesity and could be used diagnostically for risk assessment.
Published on: Oct 1, 2016 Pages: 12-18
Southeastern University, China
Peertechz Journal of Computer Science and Engineering
University of Pisa, Italy
Annals of Psychiatry and Treatment
Columbia University, USA
Journal of Novel Physiotherapy and Physical Rehabilitation
Democritus University of Thrace , Greece
International Journal of Vascular Surgery and Medicine
Dr. Pierre Guertin
Laval University, Canada
Peertechz Journal of Gerontology and Geriatric Research