Objective: The study was planned to investigate the effect of Eruca sativa seeds’ oil (ESSO) and Raphanus sativus seeds’ oil (RSSO) on impaired glucose tolerance, lipid profi le and oxidative stress in streptozotocin-induced diabetic albino rats. Also, the probable echanisms of action were suggested.
Methods: Experimental diabetes mellitus was induced by a single dose of 45 mg/kg b. wt streptozotocin. The diabetic rats were then divided into three groups: the 1st one was given 1% caboxymethylecellulose (CMC) and was considered as diabetic control, the 2nd group was treated with 50 mg/kg b. wt ESSO dissolved in 5 ml CMC and the 3rd group was treated with 50 mg/kg b. wt. RSSO dissolved in 5 ml CMC by gastric intubation daily for 30 days. The diabetic control rats were compared with normal group.
Results: The obtained data revealed that both ESSO and RSSO treatments led to a signifi cant amelioration of glucose tolerance, lipid profi le, serum insulin and liver glycogen content and the activities of glucose-6-phosphatase, aspartate aminotransferase and alanine minotransferase as well as the antioxidant defense system in diabetic treated rats. The histopathological examination of the pancreas revealed that both ESSO and RSSO increased the pancreatic islets’ size and the number of α- and β-cells within the islet.
Conclusion: the present results proved that Eruca sativa oil and Raphanus sativus oil improved glucose tolerance, serum insulin level and metabolic pathways in streptozotocin-induced diabetic rats. Raphanus sativus oil seemed to be more potent than Eruca sativa oil in improving serum insulin level and many aspects of carbohydrate metabolism and lipid profi le in the diabetic rats. The antidiabetic effects may be mediated via enhancement of the insulin secretory response, peripheral glucose uptake and the antioxidant activity as well as attenuation of the intestinal glucose absorption.
Published on: Dec 30, 2016 Pages: 34-43