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Life Sciences Group

Global Journal of Biotechnology and Biomaterial Science



Abstract Open Access
Mini Review PTZAID: GJBBS-2-108

Important Factors Influencing Protein Crystallization

Mohnad Abdalla*, Wafa Ali Eltayb, Abdus Samad, TIM Dafaalla

The solution of crystallization problem was introduced around twenty years ago, with the introduction of crystallization screening methods. Here reported some of the factors which affect protein crystallization, solubility, Concentration of precipitant, concentration of macromolecule, ionic strength, pH, temperature, and organism source of macromolecules, reducing or oxidizing environment,additives, ligands, presence of substrates, inhibitors, coenzymes, metal ions and rate of equilibration.The aim of this paper to give very helpful advice for crystallization.

Published on: Sep 30, 2016 Pages: 25-28

Full Text PDF Full Text HTML DOI: 10.17352/gjbbs.000008