In this work we
describe fabrication, characterization and possible application of FITC (fluorescine isothiocyanate) conjugated magnetite nanoparticles (MNPs) for
biomedical applications such as imaging of cancer cells. The MNPs possessed
octahedral-like geometry with almost completely dispersed distribution with
high saturation magnetization. The final FMNPs (fluorescine isothiocyanate magnetite
nanoparticles) absorption band showed 30 nm red shift towards longer wavelength
compared to bare MNP and the laser-induced fluorescence was observed at 518 nm.
The MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) results
showed that FITC conjugation diminishes the toxicity MNPs mainly due to the
reduction of surface charge. Fluorescence microscopy confirmed the uptake and
distribution of FMNPs in MCF 7 (Michigan Cancer Foundation-7) breast cancer
cells, which suggests that it can be utilized for applications such as a
magnetic fluorescent probe for bioassay.
Published on: Jun 10, 2017 Pages: 44-50