Rational prediction of pharmacological treatment options for a novel KCNH2-linked variant of the Short QT Syndrome

Congenital disorders of cardiac repolarization are associated with risk of serious arrhythmias and sudden death. The Long QT Syndrome (LQTS) is well-established to predispose towards torsades de pointes [1].


Opinion
Congenital disorders of cardiac repolarization are associated with risk of serious arrhythmias and sudden death. The Long QT Syndrome (LQTS) is well-established to predispose towards torsades de pointes [1]. The Short QT Syndrome (SQTS) is a more recently discovered condition involving abbreviated repolarization that predisposes to atrial and ventricular arrhythmias and sudden death [2]. It is characterized by short QT intervals on the electrocardiogram, frequently with tall upright T waves and by a poor rate adaptation of the QT interval: short QT intervals persist even at slow heart rates [2][3][4]. Due to the risk of sudden death, SQTS patients are often treated with implantable cardioverter defi brillators (ICDs). Mutations to genes that encode critical components of cardiac potassium channels have been implicated in the syndrome: KCNH2 in SQT1, KCNQ1 in SQT2 and KCNJ2 in SQT3 [2][3][4]. Of successfully genotyped cases, the most prevalent mutations affect KCNH2 [3]. KCNH2 (alternative nomenclature hERG: human-Etherà-go-go-Related Gene) is responsible for encoding the pore-forming protein of channels that mediate the cardiac rapid delayed rectifi er current, I Kr [5]. I Kr is vital for normal ventricular repolarization, evidenced by the fact that lossof-function mutations in hERG-mediated subunits underpin the LQT2 form of congenital Long QT Syndrome [1,5]. Gainof-function mutations in hERG-mediated subunits underpin variant 1 (SQT1) of the SQTS [2,3].
The gene mutations with the largest effects on hERG/ I Kr channel current amplitude and kinetics in the SQTS give rise to an asparagine to lysine (NK) substitution (N588K) in the external S5-Pore linker region that contributes to control of the process of hERG channel inactivation [6][7][8][9]. The N588K mutation results in a profound positive voltageshift in the inactivation of the hERG/I Kr channel; this in turn results in greater repolarizing current, timed earlier during ventricular action potentials [6,8,9], resulting in accelerated repolarization and an SQTS phenotype. hERG is a target of Class III antiarrhythmic drugs, including methanesulphonanilides such as sotalol and dofetilide and the N588K mutation was found signifi cantly to impair the ability of this class of drugs to interact with the hERG channel [6,[10][11][12].
Very recently a new SQT1 mutation has been reported involving a serine to alanine (SA) substitution (S631A) in the hERG channel pore region [13]. The index patient was screened at the age of 6 following the sudden death of a cousin and was found to have a rate-corrected (QTc) QT interval below 320 ms. She was without symptoms until experiencing syncope ten years later during physical exertion and then underwent ICD implantation [13]. Her father was asymptomatic but also possessed a shortened QTc interval (324 ms). Her sister also possessed a shortened QTc interval (340 ms) and a modest reduction in left ventricular ejection fraction (to 47%), but without other cardiac symptoms. Neither she nor the father underwent ICD implantation [13]. An asymptomatic brother did not possess a short QT interval. However, the father had a sister who had died suddenly at 30 and another sister with two sons who had died suddenly [13]. On genetic screening, the proband, her sister and father were found to have the S631A mutation, whilst her asymptomatic brother did not [13]. The index patient also possessed an SCN10A variant (R1869C), but her father did not and her mother, who did not have a short QT interval, did have the SCN10A variant [13]. This suggests that the hERG mutation and not the SCN10A variant was responsible for the SQTS phenotype in this family [13].
The identifi cation of the S631A hERG mutation in clinical SQTS is interesting in a number of respects. Although this recent report is the fi rst to implicate the mutation in clinical SQTS, the S631A mutation has long been used in basic science investigations to study the hERG/I Kr channel's P-type inactivation process [14,15]. It produces a profound Citation: Hancox  (>100 mV) positive shift in voltage-dependent inactivation of hERG current, without affecting voltage dependent activation [15]. Under action potential voltage clamp, S631A produced changes to hERG current profi le during the ventricular action potential [16], that are very similar to those reported much later for N588K [6,8,9]. It is highly likely, therefore, that mechanisms of proarrhythmia are also similar to those reported for N588K using computational modelling [17].
Perhaps most signifi cantly, there already exists a sizeable body of pharmacological literature on the S631A hERG mutation.
For example, a study from 2000 identifi ed that the sensitivity of hERG to the class III antiarrhythmic drug dofetilide was greatly attenuated by the S631A mutation, whilst that to the class Ia drug quinidine was little altered [18]. Work from this laboratory subsequently identifi ed that a second class Ia drug, disopyramide, was able to block S631A hERG channels [19], and in a subsequent side-by-side comparison of S631A and N588K, we found similarly modest attenuating effects of the two individual mutations on the potencies of disopyramide and quinidine, whilst combining the 2 mutations (which produces a greater attenuation of inactivation) had a greater effect on the actions of the two drugs [12]. Unlike sotalol, quinidine is effective in the treatment of N588K-linked SQT1 [2,3,6,10], and disopyramide has some effectiveness too [20]. Given the preclinical pharmacological data on S631A hERG channels, it is reasonable cautiously to propose that both quinidine and disopyramide may have potential value in cases of S631A hERG mutation linked SQT1, delaying repolarization and prolonging refractoriness in this setting. In principle, these drugs could be of prophylactic use in patients with this mutation in whom ICDs were not fi tted, or as an adjunct therapy in patients with such devices.