Comparative Analysis of Conventional and Thin Prep Papanicolaou Test. Technical and Economic Aspects

Citation: Giachnaki M, Athanasiadi E, Pouliakis A, Spathis A, Kottaridi C, et al. (2016) Comparative Analysis of Conventional and Thin Prep Papanicolaou Test. Technical and Economic Aspects. Peertechz J Cytol Pathol 1(1): 018-024. 018 method of sampling, preparation and evaluation of conventional cervicovaginal smears has not changed drastically. The combination of the low cost and the high levels of diagnostic accuracy contributed to the method popularity. Scientific data indicate that the periodic examination of women using Papanicolaou test leads to a reduction of mortality from cervical cancer by 70%.

The cytological findings, irrelevant of the applied method (CC or LBC), are nowadays reported and formulated according to the revised Bethesda classification system (TBS2001 system) [6,7]. The management of women according to the diagnostic categories proposed by the TBS2001 is: 1. Inadequate: in this case the Papanicolaou test should be repeated.

WNL (Within Normal Limits):
No clinical approach is required, the test should be repeated after a few years (three of five according to the applied national strategy)

Significance) or AGUS (Atypical Glandular Cells of Undetermined Significance):
The woman is requested to perform colposcopy (or cytological examination after a period).

LSIL (Low grade Squamous Intraepithelial Lesion):
The woman is requested to repeat Papanicolaou test. According to the scientific literature 25% of LSIL cases progresses to HSIL, 25% progresses to cancer and 50% regress, as this procedure takes many years.

HSIL (High grade Squamous Intraepithelial Lesion):
The woman should be treated; the applied surgical treatment is conization. After therapy the survival rate is similar to that of healthy women.

Cancer cases:
surgical treatment is performed, followed by radiotherapy; the expected survival is 5 years.
CxCa is known to be caused almost always by human papillomavirus (HPV) infection which is the commonest sexually transmitted infection worldwide. There are about 100 types of HPV virus that can infect humans. Among them, 15 are oncogenic and may cause CxCa. The improved understanding of HPV infection along with the natural history of cervical neoplasias have nowadays, resulted in the addition of the HPV DNA test along with the Papanicolaou test as ancillary test and frequently reported as a competing test. In summary, tests related to HPV lifecycle include HPV DNA typing or identification of the existence of high risk subtypes, mRNA identification of the viral E6/E7 oncogenes that are linked to oncogenic activation and immunocytochemical examinations. LBC is provided the means to perform these additional examinations.
LBC is the most widely used starting material for the detection of HPV DNA, since nucleic acid preservation is far superior to conventional cytology samples. Unfortunately, the overall accuracy for HPV detection varies greatly depending on the primer set, the reaction conditions and the enzyme used. PCR based techniques have high sensitivity, but usually suffer from false positives due to crosscontaminations and miss-priming. Specifically concerning HPVs, there were two strategies used; one that utilizes primers that are type specific resulting in increased specificity and one that uses primers that are designed for well conserved sequences of a target gene, usually L1, resulting in amplification of various HPV types with a single primer set. The latter has been widely used in detecting HPVs in cervical samples and has provided clinical evidence of the connection of HPVs with cervical cancer. More recently developed tests for HPV detection have started using the Real-time PCR platforms. Compared to conventional PCR, Real-time PCR has many advantages such as the lower detection limit, due to increased sensitivity, and the ability to use several chemistries that allow superb specificity.
One of the significant advantages of LBC is, that due to the presence of alcohols, mRNAs are adequately preserved [8]. Detection of mRNAs of the oncogenic products of HPV E6 and E7 [9], have been studied in order to identify women with higher risk to develop HSIL. From the various methods NASBA (Nucleic Acid Sequence Based Amplification) has been shown to be more specific than DNA test, more effective in identifying HSILs after treatment than repeat cytology and more accurate in identifying women with HSILs, thus reducing revisits and referral colposcopies [10][11][12]. A similar amplification method used by a commercial test (APTIMA HPV Assay, Gen-Probe, U.S.A.) has produced results that show strong correlation of a positive result with severity of the lesion [13], with more recent studies supporting that Aptima had similar sensitivity to HC2 with improved specificity [14]. However, others have found poor specificity [15]. More recently, a flow cytometry based assay (HPV Noncontact, InCellDx, U.S.A.) has been compared to HPV DNA testing versus Hybrid Capture 2 and versus CLART2 typing, with similar sensitivity and greater specificity/positive predictive value than HPV DNA testing [16][17][18]. Furthermore, flow cytometry allows discrimination and quantification of the cellular populations present in the cervical sample that in turn allows the characterization of sample adequacy [19,20].
Due to the residual material in the LBC vials, it is possible to prepare multiple slides per biological sample. This has allowed the use of immunocytochemistry (ICC). The qualitative detection of the p16 INK4A protein in cervical cytology preparation from LBCs, is one of such supplementary techniques. This ICC method has been used in the identification of women with positive high risk HPV test results or women with high grade cervical intraepithelial lesions in screening populations. In CIN, p16 INK4A has been shown to be overexpressed after the inactivation of pRb mediated by the E7 oncoprotein from high risk types of HPV. The overexpression of p16 INK4A protein is directly linked to the oncogenesis and thus has been proposed as a future biomarker. The protein expression of p16 INK4A combined with Ki67, as a commercial KIT (CINtec Plus, Roche, Switzerland), has been used to identify, with significant higher specificity to DNA detection, women with HSIL or with higher risk to develop HSIL [21][22][23].
As the performance of each screening method (CC or LBC) differs, and the associated management costs are important, the comparison of the two methods should be in multiple levels, the technical level, which addresses only performance issues and a financial level that takes into account the various associated costs. In this study we evaluate the performance of both methods in a setting involving three Greek hospitals, and carry out the cost effectiveness analysis (CEA). In addition we summarize the pros and cons of the two methods. To the authors' knowledge this is the first study in the Greek health care environment.

Study population
In order to compare the performance of the CC and the LBC, 23,604 conventional smears from the same number of women were analysed and 23,604 ThinPrep smears from equal number of different women. The smears were collected from 1/2/2006 until 31/1/2007.
The study was performed at the departments of cytopathology of a) University General Hospital ATTIKON b) "Alexandra" Hospital and c) "Agia Olga" Hospital, all located in Athens, Greece. The study was conformant to the Helsinki declaration; as there are no interventions in the participating women, there was no requirement to obtain signed informed consent forms from the study population.

Methods
The cytological findings were formulated according to the revised Bethesda classification system (TBS2001 system) [6,7]. We considered that the standard clinical approach is applied, according to each diagnostic category as presented in the background section.
In order to address the cost of the various examinations and treatments, we used the costs as suggested by the Greek National Health System, specifically: Cost of conventional Papanicolaou test: Includes the cost of woman admission, consumables, the health professional time costs, and the time required to be spent by the woman (about 4 hours). This cost was: 15.99 €.

Cost of ThinPrep Pap test:
In this case the required consumables are different, due to the use of a vial containing the liquid and the application of a technique to create single layer specimens, which requires the use of an additional filter by a device (ThinPrep Processor: ΤΡ2000). The depreciation percentage equals to 20% of the device price per year. The cost of ThinPrep Pap test was calculated to be 25.94 €, including depreciation.

Cost of treatment:
The price that insurance organizations reimburse the hospital was used as treatment cost. This cost is for colposcopy: 11.74€, for conization 103.00€ and for cancer therapy (surgical treatment, medication and radiotherapy) 3,276.4€ (mean value).

Assessment:
In order to assess the results with orientation to the outcome of the health status of women and the effectiveness of diagnosis, two parameters were considered: the calculation of years of life saved (YoLS) and the Cost to "win" a single YoLS (Cost/YoLS), being the fraction of the additional cost required for ThinPrep Pap test by the total years of life gained in the study population.
For the calculation of the YoLS, we considered the life expectancy of Greek women as it is reported by the National Statistics Institute, which is 80.7 years. As the average age of women in this study is 55 years and the life expectancy of women with CxCa is 5 years, the YoLS for every woman diagnosed at an early stage of the disease and therefore does not progress to cancer are 20.7 years.

Results
The results of the CEA are summarized in  There are several other aspects of ThinPrep cytology and in general LBC that are not considered in the cost effectiveness analysis in this study, most of them would result in even lower cost/YoLS: 1. The consumed time by cytopathologists: The average time to examine a single ThinPrep slide is 3 minutes, while the average examination time for conventional slides is 6 minutes. In the sample studied, the LBC method leaded to a reduction of 1581 man hours.

As ThinPrep vial allows additional examinations without
the need for an additional sample, there is no need to recall women; this produces additional cost savings for the patients.
3. Transportation of samples from sampling sites to the cytopathology laboratory facilities via LBC reduces errors and contributes to the reduction of pre-analytical errors. 4. The shorter turnaround time in the cytopathology laboratory and the less time needed to obtain the examination results along with the higher sensitivity comparing to conventional cytology contribute towards the confidence of the method and a higher trust level on the patient side. 5. The higher confidence level contributes as well to the reduction of the number of women who do not follow or quit the organized screening programs. 6. The LBC method introduces one more stage in the slide preparation, i.e. the use of a device that creates mono-layer slides; this process introduces an overhead of about one minute for slide preparation. This overhead is not considered in the study, however to the authors' opinion this may be surpassed as the overhead for the cytopathologists for conventional slides is about three minutes/specimen. 7. LBC vial has higher weight and volume than conventional cytology slides, thus transportation costs for the same number of samples is higher. This cost was not considered in this analysis, as samples are transferred by sample takers (midwifes) to the cytopathology laboratory on a scheduled basis. On every visit, sample takers receive the cytological results of the previous batch and submit the new samples. Additionally, many of the samples are obtained from the hospital clinics, thus there is no associated transportation cost. In the authors' opinion, additional cost for sample transportation would add a relatively small cost to the process compared to the health outcome gains.
8. Other advantages of LBC include the alcohol of the solutions which acts as a fixative for cells, inactivates microbial flora that causes lyses or red blood cells and mucus. Additionally, the pH of the solution allows the preservation of the morphological characteristics of the cells. Moreover, via LBC method, all collected cells are removed from the collection device immediately, thus dehydration and oxidization of cells are avoided as they remain exposed in the air for a very short time. The availability of the biological material in the vial allows the application of ancillary molecular methods.

Discussion and Conclusions
According to published research, false positive rates in conventional Papanicolaou test range from 5-10%. However the most severe issue remains the percentage of false negative results. According to bibliographical data, false negative cases range from 5% to50%, according to Gay et al. [26], this percentage is 20%, and in a meta-analysis study performed by the Agency for Health Care Policy Research published in 1999, this percentage was 50% [27]. Thus, quality control of the process and of cytopathology laboratories is an important issue [28][29][30], as it can increase the performance of the cytological examination.
Most of the studies agree that 60% -90% of false negative cases are due to wrong sampling of the biological material [26,31]. According to Hutchinson et al. [32], more than 80% of collected cells from the cervix are not deposited in the glass slides. Moreover, it is not possible for gynecologists to select a representative sample for the slide. This was evident in our analysis, as the percentage of inadequate samples was 23.54% and 14.64% for CC and LBC respectively, thus LBC reduced significantly (p<0.0001) the inadequate percentage by 38%. The process for conventional slides often leads to bad quality specimens due to inadequate fixation and excess blood and mucus. Thus, the microscopic examination becomes laborious, difficult and error prone as it is hard to identify rare cells indicative of neoplasias. LBC clears all these artifacts and cytopathologists examine a smaller and clearer area of the slide.
In terms of ambiguous results (ASCUS and AGUS), the outcomes of this study showed a reduction of the relevant percentage (4.80% and 2.28% ambiguous cases in CC and LBC respectively) by 53% (p<0.0001). Reduction of this percentage were reported in other studies, for example 26.59% was reported in [33] and 52.75% in [34].
Simulation experiments [35], showed that simply substituting CC via LBC without any change in compliance, may result in more than 32% in reductions of the incidence of CxCa. It has been proved that LBC is a cost effective method when applied in the general population, specifically in the Greek health system the introduction of LBC costs less than 50€ per saved life year, a cost lower than the cut off used (about 50,000$).
The cost effectiveness analysis subjects to numerous parameters, such as the incidence of the disease, the specific costs of the involved "components" including examination and treatment costs, the conformance of the population in the required repetitive process. All of these may be different from country to country and even in different regions in the same country. Future studies may include more detailed analysis for factors not being considered, for example savings due to the reduced work hours of cytopathologists and the economic effects on the women due to reduced psychological effects.
Conventional Papanicolaou test has saved and continues to save millions of women. The introduction of LBC 50 later was an important enhancement, in terms of performance as shown in this study and other existing studies, as well as for improved quality and enhanced standardization of the process. Moreover, LBC has paved the way for the application of modern molecular techniques, either as adjunctive to the test or claiming to be alternative. These modern techniques that would not be possible without LBC, have the potential to provide important outcomes for the HPV lifecycle and the deeper knowledge of the disease's natural history.