Prenatal Stress Reduces Learning and Memory in Pre-pubertal, Young, and Adult Rats of Both Sexes

Prenatal stress (PS) induced by immobilization produces defi ciencies in spatial learning and information retrieval. These defi ciencies seem to be larger in males than in females, and have been explained as an effect of fetal exposure to high concentrations of maternal corticosterone during stress response. However, the effects of PS have only been assessed at a single time point and/or sex. In this work, the effect of PS on spatial learning and memory induced by immersion in cold water was evaluated in young and adult rats of both sexes. PS was induced during gestational days 15 through 21. Corticosterone in dams, body weight, corticosterone, learning and memory were assessed in male and female offspring at one, two, and three months of postnatal life. Results showed that escape latencies of PS rats of both sexes were longer as compared to those of control groups and that the number of platformsite crossovers and time spent in the platform quadrant were lower in the PS animals as compared to the control groups. Corticosterone levels were higher in PS females and males compared with controls. The body weight was decreased only in PS males of one month of age. These results show that PS by immersion in cold water alters learning and memory processes in the offspring, regardless of sex or age, since the effects are similar in females and males during youth and adulthood. These behavioral effects are related to high serum corticosterone. Research Article Prenatal Stress Reduces Learning and Memory in Pre-pubertal, Young, and Adult Rats of Both Sexes Guerrero Aguilera María de los Angeles1, Rubio Osornio María del Carmen2, Galván Arzate Sonia3 and Retana-Márquez Socorro4* 1Posgrado en Biología Experimental. Universidad Autónoma Metropolitana Iztapalapa, México 2Departamento de Neurofi siología, INNN “Manuel Velasco Suárez”, México 3Departamento de Neuroquímica, INNN “Manuel Velasco Suárez”, México 4Departamento de Biología de la Reproducción, Universidad Autónoma Metropolitana Iztapalapa, México Dates: Received: 21 February, 2017; Accepted: 07 March, 2017; Published: 08 March, 2017 *Corresponding author: Retana-Márquez Socorro, Department Biology of Reproduction, Universidad Autónoma Metropolitana-Iztapalapa, Mexico City, CP 09340, Mexico, Tel: (52 55) 5806 4701; Fax: (52 55) 5804 4930; E-mail:


Introduction
Prenatal stress (PS) is the result of fetal exposure to the elements of stress response in dams, and has been related to cognitive deterioration in the offspring during adulthood [1][2][3]. In humans, high glucocorticoid concentrations during the fetal stage are associated with the presence of a number of psychiatric pathologies in children and teenagers, such as schizophrenia [4], autism [5,6] attention defi cit hyperactivity disorder [7][8][9], and learning and language deterioration [10][11][12].
Few of these studies account for gender differences and most observations have only been performed in male individuals [11,13]. Thus, experimental models in animals were developed to study the adverse effects of PS on cognitive functions [14].
PS deteriorates spatial learning and memory in male [15][16][17], but not in female rats [18]; in addition, PS eliminates the natural differences between sexes in cognitive function [19]. In three-month-old animals PS by immobilization [20], and two-month-old animals PS by a variety of short-term stressors (immobilization, forced swimming, and permanence on an elevated platform) [18], during the last gestational week, altered learning in male rats, but not in female, was reported. Another study showed that maternal physical stress deteriorates spatial learning and memory in rats of both sexes at 40 days of postnatal life, but maternal psychological stress only affects males [21]. Different results among studies may be explained in terms of the diverse protocols for PS used (varying in duration, intensity or gestational period) and/or the age of evaluation. Currently, there are no reports evaluating the effects of PS on learning and memory in animals of both sexes at different stages of postnatal life (prepubertal, young, and adult). Therefore, the objective of this work was to study whether PS applied during the last third of gestation alters cognitive performance in offspring of both sexes at one, two, and three months of age. Corticosterone was also evaluated in dams and offspring. We have evaluated corticosterone levels in dams exposed to stress in cold water and demonstrated that this paradigm is stressful for them [22].  [23]. This stress procedure involves the combination of two well-known physical stressors: cold (15°C) and immersion in water. Additionally, it has a psychological component of no scape possibility during the exposure. Thus, the additive components of these stressful situations, and the duration of the exposure to this stressor are enough to cause an intense response of adrenal axis, even more intense than immobilization does, as has been reported earlier [24]. Dams

Spatial navigation task
Learning and memory spatial performance of rats was measured using the Morris Water Maze (MWM) task. The MWM consisted of a circular pool, diameter of 170 cm and depth of 50 cm, surrounded by white walls. The pool was fi lled with water to a height of 30 cm and kept at a temperature of 21 ± 2 °C. The pool was divided into four equally-sized imaginary quadrants which were labeled according to the four cardinal points (NE for northeast, NW for northwest, SE for southeast, and SW for southwest) using the maze access as the reference. Three colorful pictures were set on the walls to serve as spatial clues.
A transparent acrylic platform (19 cm X 22 cm) was placed 2 cm below the water level in the NE quadrant, and equidistantly to the wall and center of the pool.
At the three different ages, one (n=10), two (n=10), and three (n=10) months old, the animals performed daily learning sessions for four consecutive days (days 1 through 4) consisting of four trials per session. Forty eight hours after the learning sessions, animals performed a single-trial session (day 6); and 24 hours later (day 7), a memory trial without the platform, allowing the rats to swim for 60 seconds, was performed. All the sessions were video recorded. Thirteen days after the beginning of learning sessions (day 13), all the animals performed a test in the maze with the platform inside.
Each trial started placing the rat on the surface of water with the snout pointing to the pool wall, in a quadrant different from the starting quadrant of previous trials. If the rat did not fi nd the platform after 60 seconds, the rat was directed by the experimenter to the platform and left on the platform for 30 seconds [25]; this procedure was only performed in the fi rst trial. When the rat found the platform within the 60-second limit, the rat was left on it for 30 seconds.
The behavioral parameters analyzed were: escape latency, defi ned as the time spent by the rat to reach the hidden platform; and, during the trials without a platform in the pool, time spent in the platform quadrant, the time spent by the rat swimming in the NE quadrant, and platform-site crossovers, being the number of times the rat swam over the specifi c former location of the platform.

Body weight registration
Body weight registration was daily performed during the fi rst three months of postnatal life. Each animal was placed on a scale to determine its weight. Once the MWM trials started, the weight registration was performed before the learning and memory sessions.

Corticosterone concentrations
Blood samples were extracted from control (n=6) and stressed (n=6) dams, at gestational day 18. Blood samples were also obtained from progeny at 1, 2 and 3 months of age, after the last test at day 13. Animals were anesthetized with pentobarbital sodium (100 mg/kg; Pisabental, México), samples were extracted by cardiac puncture into test tubes and centrifuged at 3000 X g during 25 min at room temperature. Chromatographic system calibration was performed using standards covering the range of 0-500 ng/dl for corticosterone in daily work. The regression lines between the peak heights and the concentrations of corticosterone were used for determining the unknowns. A typical regression line for corticosterone was: y = -0.6464+ 0.01655x (r = 0.99986).
Inter-assay and intra-assay coeffi cients of variation (C.V.) were determined using four plasma pools in the 15-500 ng/ dl range for corticosterone, covering the normal range in rat plasma. The intra-day C.V. ranged from 1.5 to 2%, and the inter-day C.V. inter-day was 1.4 to 3.16%.
The detection limit of the assay for corticosterone was 5 ng/ mL, using one ml of sample, at a signal to noise ratio of 2:1. The limit of quantitation for corticosterone was 10 ng/mL, and was determined with the formula: LOQ=10/s, where s is the slope of calibration curve and  the S.D. of blank response.

Statistical analysis
All the statistical analyses were performed using the SPSS statistical software. MWM differences across trials were analyzed by means of an analysis of variance (ANOVA) for repeated measures. The analyses for time spent in the platform quadrant, platform-site crossovers, and body weight were performed using one-way ANOVA. The latency means in the MWM were analyzed utilizing a t-Student test and a one-way ANOVA, followed by the Newman-Keuls post-hoc test. The analysis of the percentage of animals that failed to found the platform site was performed using a Chi-squared test. Data were considered signifi cant when p < 0.05.

Serum corticosterone concentrations in pregnant females
Serum levels of Corticosterone in stressed pregnant rats were signifi cantly higher than those in control pregnant females (t = -5.127, p < 0.0001) at day 19 of pregnancy ( Figure  1).

Effects of prenatal stress on spatial learning and memory
One-month-old PS females showed escape latencies signifi cantly longer in days 4 and 6 of MWM training as compared to latencies of the corresponding female CON (F (5, 44) = 4.82, p < 0.048622). The mean escape latency of PS females was signifi cantly longer as compared to the mean escape latency of the respective CON females (t = -3.8703, p < 0.000217; Figure 1). The escape latencies of the one-month-old PS males on days 1, 3, 4, and 13 were signifi cantly longer than latencies of the corresponding CON males (F (5,44) = 6.89, p < 0.021003). PS animals swam around the maze most of the time. The mean escape latency of PS males was signifi cantly longer than the mean of the respective CON males (t = -3.9713, p < 0.000146) (Figure 2).
Escape latencies in two-month-old PS females were signifi cantly longer on days 3, 4, 6, and 13 during MWM training (F (5, 44) = 5.55, p < 0.03152). The mean escape latency of PS females was signifi cantly longer as compared to that obtained by the CON group (t = -4.2937, p < 0.000039). The two-month-old PS males showed signifi cantly longer escape latencies as compared to the respective CON males on days 1 through 3 of the MWM training (F (5, 44) = 8.49, p < 0.010142).
PS animals swam around the maze most of the time also. As well, the mean escape latency obtained by the PS males was statistically longer than that obtained by the respective CON males (t = -3.9082, p < 0.000164) (Figure 3).   =8.5714) PS females was signifi cantly larger as compared to the respective CON females. There were not differences between groups of males that did not reach the platform (Table 1).

Corticosterone concentrations in female and male offspring
The levels of corticosterone in PS female (F (3, 42) = 11.495, p < 0.00001) and male (F (3, 42) = 8.762, p < 0.0001) offspring at the end of MWM tasks (day 13) were signifi cantly higher than in control offspring at all ages evaluated ( Figure 6).

Body Weight:
Only the one-month-old PS males showed body weights signifi cantly lower than the CON males (F (5, 94) = 4.80, p < 0.01434). In the two-and three-month old PS males, there were no differences between the body weights of PS and CON. There were not differences in females at any age ( Figure   7).

Discussion
The results of this work demonstrate that stress by immersion in cold water causes disruption in learning and memory in offspring during postnatal life. The increase in glucorticoids during pregnancy due to stress can cross the placental barrier as well as fetal blood brain barrier, and can modify the function of fetal brain structures such as brain cortex and the hippocampus [26], and these alterations can disturb behaviors such as learning and memory in the offspring [12,22]. Indeed, the results obtained in this work show that PS Figure 3: Prenatal stress (PS) effects on spatial learning and memory in twomonth-old rats. PS female rats obtained longer escape latencies on Days 3, 4, 6, and 13 as compared to the control (CON) females. PS males obtained signifi cantly longer escape latencies on Days 1, 2, and 3 of training as compared to the CON males. PS females and males obtained signifi cantly longer escape latencies as compared to the CON animals. Data shown as X ± SEM. * p < 0.05, as compared to the respective CON groups.

Figure 4:
Prenatal stress (PS) effects on spatial learning and memory in threemonth-old-rats. Female and male PS rats obtained longer escape latencies for all the training days as compared to the control (CON) animals. PS females and males obtained signifi cantly longer mean escape latencies as compared to those of the CON animals. Data shown as X ± SEM. * p < 0.05, as compared to the respective CON groups.  indicates that PS rats are not capable of using spatial cues to fi nd the platform. These results differ from previous reports where female and male rats PS stressed by immobilization and bright light [27], or using a stressor battery (immobilization, forced swimming, and elevated maze) [18,20,28], neither PS females nor males showed defi ciencies in spatial learning. These differences may be explained by the type of stressor used in this study, due to its larger intensity as compared to other stressors [24]. Other studies report that PS induced by a physical stressor affects one-month-old rats of both sexes, but psychological stressors only affect males [21]. These authors suggest that increased cognitive ability in PS females as compared to that in PS males may be explained by the neuroprotective effect of estrogens in females, which makes females less susceptible to present cognitive disorders. In addition, estrogens increase the number of dendritic spines, long-term potentiation (LTP) and N-methyl-D-aspartate (NMDA) receptors in the hippocampus and infl uence cholinergic transmission in the forebrain, which promotes learning and memory in females [29,30]. The aforementioned supports the hypothesis that estrogens may be protecting brain structures involved in learning and memory of spatial information in PS females when the immobilization technique is used. Furthermore, it has been shown that prenatal stress by immobilization does not alter the estrous  cycle of female rats [31] or estrogen levels during adulthood [32]. In this study, PS was induced by immersion in cold water, which is categorized as a high intensity stressor producing lager activation in the adrenal axis as compared to stress by immobilization [33]. We have observed that PS by immersion in cold water may alter the progression of the estrous cycle and diminish the plasmatic concentration of estradiol (data not published). Therefore, the changes in cognitive performance of PS females found in this work may be explained by the exposure to high concentrations of maternal corticosterone observed, alterations in the adrenal axis of the offspring, showing high concentrations of corticosterone during adulthood, as has been reported previously [22], as well as low estrogen concentrations. PS produces hyper activation of the adrenal axis during adulthood because basal concentrations of corticosterone in PS are larger than those observed in non-PS animals [22]. Furthermore, the adrenal axis in PS animals is hyper reactive because of the elevated concentrations of corticosterone as compared to those of non-PS animals before novel and stressful situations [33][34][35][36] and such concentrations even take longer to return to basal levels after the exposure to stressors [37]. These alterations in the adrenal axis can importantly contribute to deterioration of spatial learning [38]. The results of this work show that corticosterone levels are signifi cantly higher in PS animals compared with controls. These results are similar to those reported previously in our laboratory showing that three-month-old PS rats of both sexes present defi ciencies in spatial learning, and increase in the concentration of serum corticosterone after performing the MWM task [22]. Therefore, cognitive defi ciencies in the PS offspring of both sexes in the three ages studied herein may be due, at least in part, to the high concentrations of corticosterone produced by the stress response (adrenal axis hyper -reactivity) resulting from the exposure to the MWM task. Learning defi ciencies in the offspring PS by immersion in cold water could also be a consequence of decreased plasticity in the hippocampus of these animals. Additionally, hippocampal CA1 presents a decrease in LTP, which can be apparent until adulthood [39]. These results suggest that PS may deteriorate learning ability during youth and adult stages independently on the sex of the individual.
In this study, the retrieval ability of the PS offspring to perform the MWM task was evaluated. Escape latency and platform-site crossovers were measured 48 hours and one week after the fourth training day. One-and three-month-  [20,40], but this effect is not observed in twomonth-old males [18]. There are few reports of follow-up until adulthood in which females are included. In this work, PS female rats also showed defi cits in the ability to remember spatial information at the three ages analyzed and in both sexes. Learning and memory differences in PS animals of this work, as compared to other studies, are probably due to the type of stressor used and its intensity. Immobilization is a stressor of mild intensity and its repeated application produces habituation. This mild-intensity stressor, applied in rats, results in a robust initial secretion of corticosterone, which gradually decreases through the days of exposure to the stressor [24,41,42]. The stressor by immersion in cold water does not produce habituation, as demonstrated in a study where rats exposed to this stressor during 20 sequential days presented corticosterone concentrations increased by 3.7 times the normal concentrations, which also remained increased along all the stressor-exposure period [24]. Thus, we propose that high corticosterone concentrations in fetal serum during the last gestational period are produced by the exposure to the stressor by immersion in cold water, also altering brain development, and learning and memory processes. Hence, effects of PS by immersion in cold water on spatial learning Figure 7: Body weight in prenatally stressed (PS) females and males at three different ages. One-month-old PS males showed lower body weight as compared to the control (CON) animals. Data shown as X ± SEM. * p < 0.05, as compared to the corresponding CON group.
and memory in the animals of both sexes studied herein may result from the exposure to high concentrations of corticosterone during fetal development. The hippocampus is a brain structure involved in processing of spatial information and it is one of the main structures targeted by corticosteroids during neonatal brain development. Various studies report that PS animals show diminished dendritic arborization, dendriticspine density and synapses [43][44][45]. There are reports of decrease in dendritic segments and arborization of granular and pyramidal cells in the hippocampal CA3 region [46]. Even more, PS produces reduction in cellular proliferation, brain-derived neurotrophic factor (BDNF) [47], neurogenesis, and total hippocampal volume [15,48]. In a previous study, we reported that PS causes decreased neurogenesis in the hippocampus of males but not in females, which correlates with learning and memory defi ciencies. This decreased neurogenesis correlates as well with increased corticosterone levels in PS animals [22], which can explain the defi ciencies in learning and memory observed in PS females. Therefore, the results of this work can be explained by reduced hippocampal neurogenesis in both females and males Body weight was lower only in one-month-old PS males, compared to the corresponding CON animals, which were later recovered to the normal. PS females did not show differences at any age. These results are different from other reports where no alteration in body weight were observed in males or females as an effect of PS from day 20 through 80 of postnatal life [19]. Data from this work suggest that PS by immersion in cold water may infl uence body weight, depending on their sex and age, being one-month-old males the most vulnerable to its effects.

Conclusion
In conclusion, this work shows that prenatal stress induced by immersion in cold water disrupts the learning and memory processing of spatial information in the offspring of both sexes, and these defi ciencies persist from infancy to adulthood. These defi ciencies could be related, in part, to the high levels of corticosterone observed in male and female offspring. Prenatal stress alters body weight of male rats during childhood, as well.