Colonic perforation in 91- year- old man with severe Covid-19 infection

Citation: Pyar KP, Shan MA, Hlaing SW, Diwon, Aung ZH, et al. (2020) Colonic perforation in 91yearold man with severe Covid-19 infection. J Clin Microbiol Biochem Technol 6(1): 041-043. DOI: https://dx.doi.org/10.17352/jcmbt.000043 (1262 U/L), high CRP (297.94 mg/l) and serum creatinine was marginally raised (1.6 mg%) Thus, he was treated as a case of severe Covid-19 infection with oxygen 10L/min, imipenem, azithromycin, dexamethasone, low molecular weight heparin, vitamins and dexamethasone. Convalescent plasma therapy was given on Day 12 symptom onset.


Introduction
The novel coronavirus disease, caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has spread world-wide since 2019 December. Over 62.5 million people have Covid-19 infection and 1.44 million died from severe infection [1]. The mortality rate is also high in elderly [2], those having co-morbid states like diabetes, hypertension, heart failure, COAD, end stage renal disease, immunosuppressant therapy. The cause of death is ARDS, multi-organ failure and pulmonary embolism. Although gastrointestinal symptoms are not uncommon, colonic perforation is very rarely reported.

Clinical presentation
A-91-year old man had fever, cough with dyspnoea for 7 days and was brought to hospital. He was previously healthy and lived with caring family. He did not have dementia and was ambulating well. He was not diabetics nor hypertensive.
His nasopharyngeal swab for Covid-19 PCR was positive. His SaO2 on air was 83% and respiratory rate was 28/minutes.
Chest Xray was consistent with severe pneumonia as patchy opacities occupied more than 50% of lung parenchyma on both sides (Figure 1(a)). His total WBC was 31.6 X10 9 /l and absolute lymphocyte count was 2.2 X10 9 /l. He had high LDH

Case summary
A-91-year old man had severe Covid-19 infection (bilateral pneumonia, SaO2 85% on air). He was treated with antibiotics, oxygen, dexamethasone and convalescent plasma therapy. On day 28 symptom onset, he noticed abdominal distension without abdominal pain. CT abdomen revealed pneumoperitoneum. An area of perforation at colon with mucosal haemorrhage was seen in laparotomy. Right hemicolectomy was done. Histology showed mucosal oedema, infi ltration with infl ammatory cells mainly polymorphs and lymphocytes, congested vessels with areas of haemorrhage in the mucosa.

Discussion
Covid -19 infection has over all mortality rate of 5% in all age group and it is high in older age, those having comorbidities like diabetes, hypertension, heart failure, COAD, end stage renal disease, immunosuppressant therapy [2]. In this case, apart from very old age of 91 year, there is no other medical diseases.
Several studies mentioned that gastro-intestinal manifestation is found in 10-20% of Covid-19 infection [3,4]. One of the reports mentioned identifi cation of Covid-19 virus in the mucosa of gastrointestinal tract. They proved that SARS-CoV-2 RNA detection and intracellular staining of viral nucleocapsid protein in gastric, duodenal, and rectal epithelia in patients with Covid-19 infection [5].
SaO2 was 95% on air on Day 21 symptom onset. His appetite was apparently good and he was on normal diet. His antibody level after plasma therapy was better. IgG level rose from 4.33 mg/dl to 16.46 mg/dl and IgM level from 1.02 mg/dl to 1.68 mg/dl. LDH level dropped (358U/L) and CRP also (58 mg/l). His total WBC became 16.89 X10 9 /l. But absolute lymphocyte count dropped i.e., 0.33 X10 9 /l. On Day 24 symptom onset, he had loose motion (2 to 3 times per day). There was no abdominal pain or vomiting. There was no blood or mucus in stool. Then he noticed abdominal distension gradually and discomfort. On Day 28 symptom onset, there was features of peritonism on examination. CT abdomen revealed massive free gas and oedematous colon on right side ( Figure 2). Later, he developed fever and blood pressure dropped. Inotropes, metronidazole and fl uid and electrolytes were given.
Laparotomy was done under general anaesthesia. There was free air and a small area of perforation (0.8 mm) in ascending colon. On cut section, mucosal congestion with submucosal haemorrhagic areas were seen. There was no growth or ulcer. There was no pseudo-membrane ( Figure 3).
Right hemicolectomy and peritoneal toileting were done. Drainage tube was inserted. Histology showed mucosal oedema, infi ltration with infl ammatory cells mainly polymorphs, lymphocytes, few plasma cells and histiocytes. Congested vessels with areas of haemorrhages in the mucosa (Figure 4). Organisms, granuloma, cellular and nuclear atypia are not found.
Both intra-operative and immediate post-operative period were very critical. His SaO2 dropped and ventilatory support, two inotropes, blood transfusion and fl uids were given. Then, features of ARDS set in ( Figure 5). Two days later, his condition deteriorated more and expired.   An atypical manifestation of colonic perforation in patient with Covid-19 infection was mentioned in one report. The patient was a-80-year old female. She presented with both respiratory and abdominal symptoms. And she had pneumothorax and acute perforated abdomen [6].
The report from Italy also mentioned a case of colonic perforation which was diagnosed on 10 day of hospitalization. The patient was a -53-year old male, hypertensive with severe Covid-19 infection, presented with multi-organ failure [7]. The fi ndings in CT abdomen was similar to our case.
Another case was a -34 -year man, obese (BMI 36), with severe Covid-19 infection. He got occult colonic perforation following interleukin -6 receptor antagonist therapy. They identifi ed SARS-CoV-2 virions in the colon by electron microscopy [8]. In their description, abdominal examination did not reveal features of peritonitis i.e., similar to initial fi ndings of our case. The intestinal complication involving both small and large intestine was also recognized in a patient with Covid-19 infection who received treatment with tocilizumab. He was 43 year and he had ulceration in ileum with colitis and ileal perforation [9]. They assumed that the possible relationship between even a single dose of tocilizumab and gut ulceration in Covid-19 populations needs further investigation. [9]. In our case, the patient did not receive interleukin -6 receptor antagonist therapy.
In Covid-19 infection, elderly population with comorbidities are found to develop more complications [2]. Thus, their mortality is higher than others. L Marco and et al highlighted acute intestinal manifestations in Covid-19 patients and they pointed out the awareness among them. There are other reports too [10]. Kangas-Dick, et al. [11] also reported a case of duodenal perforation in Covid-19 case with severe pneumonia.
The possible mechanism of injury is interesting. One way is direct infi ltration of colonic cells by Covid-19 virus. It was proved in earlier study where they found Covid-19 virus in rectal mucosa in addition to oesophagus and gastric mucosa [5]. In this case, we did not do viral RNA in histological specimen or stool. Another mode of injury is due to autonomic neuropathy of colon as a result of Covid-19 related neuronal injury [7,12].

Conclusion
In Covid-19 pandemic era, though the common presentation is mainly fever, cough and dyspnoea, awareness of abdominal symptoms is also important. It may be initial presentation or late -28 days symptom onset in this case. Colonic perforation is a very rare complication. It may develop as a result of direct viral invasion or virus-related neuronal injury causing colonic stasis. It may be due to complication of interleukin-6 receptor antagonist therapy.