Glaucoma: May new technologies help in early diagnosis?

Purpose: to identify new functional parameters that may help in the early primary open angle glaucoma (POAG) diagnosis. Materials and Methods: 40 subjects were enrolled: 20 healthy subjects and 20 POAG patients, all aged between 30 and 65 years, for a total of 79 eyes, including 40 healthy and 39 POAG. All subjects underwent microperimetric test, Nidek MP-1 (NAVIS, software version 1.7.6; Nidek Technologies, Japan) using Humphrey 10-2 pattern with manual polygonize of upper and lower hemifi elds. Mean hemifi eld sensitivity was evaluated. Results: Analysis reported signifi cant differences of mean retinal sensitivity of the upper hemifi elds between POAG and healthy subjects (p < 0.025), thus highly signifi cant. Equally signifi cant (p < 0.025) was the comparison of ratio (upper / lower hemifi eld) between the two groups. There was not statistically signifi cant difference of sensitivity of lower hemifi elds (p> 0.05). Conclusion: signifi cant difference was observed between healthy and POAG groups using Humphrey 10-2 vertical asymmetry index. Therefore, it appears to be an unprecedented microperimetric diagnostic tool. Research Article Glaucoma: May new technologies help in early diagnosis? Casillo L, Tricarico S and Vingolo EM* La Sapienza University of Rome, Ophthalmology Department, Terracina, Italy Received: 19 February, 2018 Accepted: 15 March, 2018 Published: 16 March, 2018 *Corresponding author: Vingolo EM, La Sapienza University of Rome, Ophthalmology Department, Terracina, Italy, Tel: +393486500312; E-mail: https://www.peertechz.com


Introduction
Glaucoma is a degenerative optic-nerve disease, characterized by progressive retinal ganglion cells loss that leads to visual fi eld functional defi cit and typical optic nerve head alterations. High intraocular pressure (IOP) represents the main risk factor. Untreated glaucoma can lead to a severe loss of visual capacity, due to a total loss of peripheral visual fi eld and, in 15% of cases, it degenerates in blindness [1]. Nowadays 90 million people in the world are suffering from glaucoma, of which 9 million in Europe [2].
High intraocular pressure (IOP) is the most important factor for onset and progression of optic nerve degeneration in glaucoma patients. Optic nerve head degeneration in glaucoma is characterized by progressive retinal ganglion cells and their axons loss, due to an apoptotic process, whose trigger, even today, remains matter of debate.
Current therapies are able to delay or, at best, to stop disease progression. However, once a ganglion non-reversible apoptotic damage occurred, it will be not possible to fully restore the functional defi cit.
About that, it is highlighted the importance of identifi cation of new functional parameters that can be used as markers of suffering ganglion cells, anticipating the occurring of nonreversible apoptotic damage.
Perimetry is the most commonly used functional test in glaucoma diagnosis and follow-up. This test examines the visual fi eld, i.e. the region of space visible from a steady eye in the primary position. Visual fi eld test provides parameters to defi ne functional damage severity. Computerised perimetry is mainly applied in glaucoma diagnosis and follow-up.
Microperimetry is a functional test that combines a standard computerised perimetry with retinography, providing a fundus perimetry. This technique allows us to study fi xation and retinal sensitivity threshold, setting exactly retinal spots that are stimulated by a system implemented with eye movements tracking.
The main advantages of microperimetry are the possibility to obtain accurate fundus tracking during test execution, to study fi xation stability and the possibility to overlay the sensitivity map with a retinography [3]. Moreover, thanks to eye tracking system, data are independent of eye movements and they are exactly related to the stimulated areas. This result DOI: http://doi.org/10.17352/2455-1414.000045 cannot be obtained with standard computerised perimetry [4]. Typically, glaucoma damage, on the microperimeter, consists in decreased retinal sensitivity of macular area and, especially, of peripapillary area, in direct correlation with anatomical RNFL damage [5]. Sensitivity of MP-1 microperimeter in detecting subjects suffering from glaucoma, strongly correlates with standard Humphrey 10-2 perimetry [6]. In 2012, a study reported that MP-1 has a greater predictability than the standard Humphrey 10-2 perimetry [7].
In last years, research has been directed toward identifying the most effective test to screen at-risk subjects in order to detect those patients requiring an early treatment.
Thus, our study aims to identify new functional parameters that can help early POAG diagnosis.
According to the latest research trends, we carried out a microperimetric study dividing the whole fi eld in two hemifi elds, upper and lower, with an approach that had never been reported in literature.

Materials and Methods
Between March 2015 and July 2015, at Division of Ophthalmology, "La Sapienza University of Rome" (" A. Fiorini" Hospital; Terracina, Italy), 40 subjects were enrolled (18 males and 22 females), of which 20 suffering from POAG (13 males and 7 females) and 20 healthy subjects (5 males and 15 females). Mean age of POAG group is 56.9 years. Mean age of control group was 53.7 years. The difference of ages between the two groups was not statistically signifi cant (p = 0.15) ( Table  1).
According to ICD-9 classifi cation, mild or early stage glaucoma is defi ned as optic nerve abnormalities consistent with glaucoma but no visual fi eld abnormalities on any whiteon-white visual fi eld test, or abnormalities present only on short-wavelength automated perimetry or frequency-doubling perimetry.
Inclusion criteria for early stage POAG patients were: IOP higher than 21mmHg in at least two separate measurements with Goldmann applanation tonometry corrected according to pachymetry; open iris -corneal angle at gonioscopy; no visual fi eld abnormalities on any white-on-white visual fi eld test, or abnormalities present only on short-wavelength automated perimetry or frequency-doubling perimetry; at least one RNFL quadrant out of limit values ; topical b-blockers or prostaglandins monotherapy in both eyes; refractive error not exceeding + 3,00D and -3,00D; corrected visual acuity at least 10/10 and smallest font in the near vision ; age between 30 and 65 years. In POAG group, one patient showed a unilateral retinopathy, so that one eye was excluded from our study. Therefore we studied a total of 79 eyes: 39 POAG eyes and 40 healthy eyes.
It was used a Nidek MP-1 (NAVIS, software version 1.7.6; Nidek Technologies, Japan) microperimeter , consisting of an infrared fundus camera, a second color fundus camera (CCD camera progressive scan color, resolution 780 x 580 pixels, equipped with xenon fl ash) and a PC with NAVIS operating system for data acquisition and processing.
IR fundus camera is equipped with a lens system for patient's refractive defects correction, in a range of -12.5 / +16 diopters, so that it is possible to obtain a more accurate functional assessment and a proper focus the retinal images.
Through the color fundus camera, it is possible to take snapshots of fundus and to obtain a high quality digital picture. Functional sensitivity threshold tests can be executed directly, using the color retinography as landmark, overlaying with the sensitivity map obtained through IR fundus camera.
Overlay is performed by a semiautomatic system, which places two landmarks on particular retinal areas; usually, it takes as reference the branches of superfi cial retinal vessels that offer a higher contrast on retinal structures. Test running is shown on a LCD display and directly controlled by the software.
For stimulation is used a 20 dB scale of attenuation, with maximum brightness of 400 asb. Target light is usually set at 100 asb. MP-1 also offers fi ve sizes of targets that correspond to the I to V of Goldmann perimeter. We used a target size equivalent to Goldmann III.
To examine enrolled subjects, we used the Humphrey 10-2 pattern, projecting 68 stimuli in 20 central degrees and 200 ms projection time.
Subject was seated in a dark room and was instructed to place the head on the chin rest and to press the button every time a light stimulus of varying intensity is seen. One eye at a time is examined, covering the other one. No mydriatic drugs

Statistical analysis
For the descriptive statistics we used quantitative and qualitative variables analysed using a general linear model.

Results
Analysis of the 40 enrolled subjects reported signifi cant differences of mean retinal sensitivity of the upper hemifi elds between POAG and healthy subjects (p < 0.025), thus highly signifi cant (Table 2).
Equally signifi cant (p < 0.025) was the comparison of ratio (upper / lower hemifi eld) between the two groups (Table 2).
Instead, there was not statistically signifi cant difference of sensitivity of lower hemifi elds (p> 0.05) ( Table 2).

Discussion
Data obtained with Nidek MP-1 using Humphrey 10-2 pattern and polygonize, showed that retinal sensitivity is higher in healthy group compared to POAGs, confi rming that glaucoma leads to retinal sensitivity decrease [4].
In lower hemifi elds, difference between the control group  [10].
However, to date, it has not ever been reported any study adopting our protocol.
Our vertical asymmetry index, obtained through hemifi elds polygonize on Humphrey 10-2, had never been described before.
Therefore, it appears to be an unprecedented microperimetric diagnostic tool.

Conclusion
Microperimetry is confi rmed as useful test in detecting glaucoma functional damages, especially using hemifi eldtests.
Moreover, it provided an innovative vertical asymmetry index, potentially useful in early POAG diagnosis.