A Young Lady with ANA negative SLE with Secondary Anti Phospholipid Syndrome

Systemic Lupus Erythematosus (SLE) is a chronic, inflammatory, autoimmune, multisystem connective tissue disease characterized by various autoantibodies to nuclear and cytoplasmic antigens and commonly affects the joints and a variety of organs due to an over activation of the body's immune system. There is wide heterogeneity in presentation of SLE patients, including lung, central nervous system, skin, kidney, and hematologic manifestations. The presence of antinuclear antibodies (ANA) in serum is generally considered a decisive diagnostic sign of SLE. However, a small subset of SLE patients who had the typical clinical features of SLE was reported to show persistently negative ANA tests. Our report describes a 24-yr-old female who presented with the clinical manifestations of SLE such as malar rash, photosensitivity, arthritis, oral ulcer and proteinuria. The serum autoantibodies were all negative except anti ribosomal P. She was also positive for lupus anti-coagulant. She was treated with oral prednisolone, hydroxychloroquine with topical tacrolimus and improved significantly. Three months after, repeat ANA and anti-ds DNA showed persistent negativity but lupus anti-coagulant remained positive. This case suggests that ANA may not be required in the pathogenesis of SLE.


Introduction
Systemic Lupus Erythematosus (SLE) is a complex disease characterized by an autoantibody response to nuclear and cytoplasmic antigens. The autoantibody response is associated with the inflammatory cascades and end-organ damage in the kidney, skin, brain and other organs. Especially in the kidney, immune-complex deposits of autoantibodies have been implicated as major pathogenic mediators [1].
The diagnosis of SLE can be made by fulfilling the revised criteria of the American College of Rheumatology [2]. One of the laboratory hallmarks of SLE is the presence of Antinuclear Antibodies (ANA) in the serum [3]. On the other hand, a small subset of SLE patients with the typical clinical findings of SLE was reported to have persistently negative ANA test. These patients were designated as ANAnegative SLE and their clinical picture was notable for the high incidence of photosensitive dermatitis and the low incidence of nephritis and neuropsychiatric manifestations [4]. ANA negative SLE was first introduced by Koller et al. with five cases with clinical features similar to SLE [5]. Here we report a young lady presented polyarthritis which was complicated with photosensitive rash and oral ulcer and later diagnosed as ANA negative SLE.

Case report
A 24 years old pleasant, unmarried, service holder, muslim lady admitted into Ad din Women's Medical College and Hospital with the complaints of pain in the multiple joints of both upper and lower limbs for last 2 months involving mainly small joints of hands ( Figure  1) and feet, wrists, ankles. Joint involvement was symmetrical and non-migratory. There was associated inactivity stiffness that lasted for more than an hour and relieved partially with activity and taking analgesics. Joint pain

International Journal of Clinical Rheumatology
Case Report A young lady with ANA negative SLE and Secondary Anti Phospholipid Syndrome Systemic Lupus Erythematosus (SLE) is a chronic, inflammatory, autoimmune, multisystemconnective tissue disease characterized by various autoantibodies to nuclear and cytoplasmic antigens and commonly affects the joints and a variety of organs due to an over activation of the body's immune system. There is wide heterogeneity in presentation of SLE patients, including lung, central nervous system, skin, kidney, and hematologic manifestations. The presence of Antinuclear Antibodies (ANA) in serum is generally considered a decisive diagnostic sign of SLE. However, a small subset of SLE patients who had the typical clinical features of SLE was reported to show persistently negative ANA tests. Our report describes a 24-yr-old female who presented with the clinical manifestations of SLE such as malar rash, photosensitivity, arthritis, oral ulcer and proteinuria. The serum autoantibodies were all negative except anti ribosomal P. She was also positive for lupus anti-coagulant. She was treated with oral prednisolone, hydroxychloroquine with topical tacrolimus and improved significantly. Three months after, repeat ANA and anti-ds DNA showed persistent negativity but lupus anti-coagulant remained positive. This case suggests that ANA may not be required in the pathogenesis of SLE.
Keywords: antinuclear antibody • anti-ribosomal P antibody • systemic lupus erythematosus • anti-phospholipid syndrome was associated with low grade intermittent fever (max recorded temperature was 100 0 F), not associated with chills and rigor and subsided after taking anti pyretic. Joint pain was not associated with any redness or increased warmth of the joints. She also denied any low back pain, buttock or groin pain, sole pain or pain over heel. There was also no H/O sexual exposure, urethral discharge or bloody diarrhoea preceding the illness. No red eye, visual impairment, proximal muscle pain or stiffness, papulopustular or acne form or scaly skin lesions were present either. There was also no history of dry mouth or dry eye, loss of scalp hair, venous thrombosis, tightening of skins of body, dysphagia, heart burn, altered bowel habit or any history suggestive of Raynaud's phenomenon. She denied any anorexia, night sweat or weight loss. After about one month later she developed painless oral ulcers ( Figure 2). But there were no genital ulcers.
At the same time she also developed erythematous, raised, painful, non-pruritic photosensitive rashes all over her face sparing the nasolabial folds ( Figure 3). But there were no bullous lesions or no crusts.
On further query, she stated that her paternal aunt had SLE. She had no significant drug history prior the illness except she took ceftriaxone for 7 days before admission. Her menstrual history was also noncontributory. She had neither any sexual promiscuity nor had any history of tuberculosis or any contact with the patient with active tuberculosis. On examination she was ill looking, febrile (temperature 100°F) with stable vitals. She was moderately anemic but non-icteric and non-cyanosed. There were erythematous, raised, tender rashes over both cheeks, forehead (sparing naso labial fold), there were some rashes over dorsum of hands and feet too. Skin was not thinned with nobruising or telangiectasia. Eyes including both fundi were normal, lymph nodes were not palpable, and thyroid gland was not enlarged. There was no edema, bony tenderness or any nail or periungual vasculitic changes. On musculoskeletal system examination, GALS screening was done. Gait and spine examination revealed no abnormalities. On upper limb examination, MCP, PIP and wrists of both sides were swollen, tender and there was painful restriction of both active and passive movements of these joints. DIP's were spared. On lower limb examination, ankles and small joints of feet of both sides were swollen, tender and there was painful restriction of both active and passive movements of these joints. There were neither any deformities nor any evidences of sacroilitis or enthesitis. Oral cavity examination revealed painless ulcers with erythematous irregular margin over hard palate, right inner cheek. But there were no organomegaly. There were no evidences for serositis (ascites, pleural or pericardial effusion). Other systemic examination was noncontributory.

Case Report
Gomes RR, et al.
A young lady with ANA negative SLE and Secondary Anti Phospholipid Syndrome on the patient's clinical and laboratory abnormalities [2]. Various kinds of autoantibodies are present in the sera of SLE patients, and ANA is a diagnostic hallmark for SLE, having a frequency of 95% or greater in SLE patients [6] and its titre is one of the key diagnostic criteria for SLE. But ANA positivity is not mandatory for the diagnosis [7]. Sero-negativity in lupus patients may be due to technical failure or entrapment of ANA in circulating immune complexes. One explanation for the ANA-negative finding is technical inaccuracy. McHardy et al. identified 38 adults who had a high DNA-binding capacity, but negative fluorescent ANA testing (with a rat liver substrate), and the clinical diagnosis of SLE was established for these patients [12]. In another study, the previously ANAnegative finding, with using mouse liver substrate, in the sera of patients with SLE or subacute cutaneous lupus erythematosus was found to be anti-Ro antibody positive by performing enzyme-linked immunosorbent assays [13]. Actually, the increasing use of Human epithelial (HEp-2) substrate has increased the sensitivity of ANA assays and as a result, the perceived incidence of ANA-negative SLE has decreased [12,14].
Another cause of ANA-negative findings is that ANA is present, but it's bound in the form of immune complexes. This has been described in five patients with lupus During hospital stay as patient developed high grade temperature, neutropenic sepsis was suspected and she was treated with combined piperacilin/tazobactam and aminoglycoside along with isolation and neutropenic diet. 2 units of whole blood were transfused. Systemic steroid (oral prednisolone 1 mg/kg/day) with DMARD (hydroxychloroquine) was started with topical steroid and tacrolimus ointment. Regular monitoring of both hematological and biochemical parameters were done. On discharge, Hb%-10.1 gm/L, Total count-5200/ cmm (PMN-68%, L-30%), ESR-34 mm in 1 st hr, TPC -192000/cmm, CRP-4.92 mg/dl, Urine R/E-no protein, no casts, no RBC, no WBC, S. Ferritin-243 ng/ml. After three weeks, on OPD follow up, tapering of systemic steroid was started, topical steroid and tacrolimus was stopped. She was advised to use sunscreen regularly. She was also explained about the course of the disease, treatment options, recognition of flares, and pregnancy outcome. Last but not the least, reassurance was given.
After three months, ANA, Anti ds DNA and lupus anti-coagulant were repeated. ANA and anti-ds DNA showed persistent negativity and lupus anti-coagulant remained positive. So she was started low dose aspirin with hydroxychloroquine and tapering dose of steroid.

Discussion
Systemic lupus erythematosus is a chronic inflammatory autoimmune disease that involves many different organ systems, and this illness exhibits a wide spectrum of clinical manifestations. The diagnosis of SLE depends nephritis whose ANAs, which were primarily reactive with DNA, were not detected in the serum by indirect immunofluorescence until the ANAs were dissociated from circulating immune complexes [15]. Loss of ANA through the kidney in a patient with profuse proteinuria has been reported as another possibility. In that case, the tests for ANA became positive upon clinical recovery [16]. However, most ANA-negative patients have persistently negative tests for ANA after a long followup period. Technical factors or prozone effects have been described as the possible reasons for this [17].
SLE is still a disease with significant morbidity and mortality. More recent studies have shown that latest 5 year survival is now nearer 90-95% and those 70-85% patients have 10 year survival [18]. In most studies, patient with renal involvement had poor prognosis compared to patient without it.

Conclusion
Our patient fulfilled the ACR criteria r without serologic evidence of SLE at presentation. During the follow-up period, the repeated ANA and anti-dsDNA tests were all negative, irrespective of her improving proteinuria, joint and mucocutaneous symptoms. Our patient may be a very rare case of ANA-negative SLE without seroconversion and this suggests that ANA may not be required in the pathogenesis of SLE. If patient fulfil criteria for SLE despite seronegativity, diagnosis should not be delayed as 5-10% of SLE patient are seronegative. Auto-antibodies against ribosomal P proteins appear highly specific for SLE found in 10-15% patients with SLE; therefore, they can be used as diagnostic marker for the disease. Furthermore, association has been described with particular manifestations of lupus, especially with neuropsychiatric, renal, and hepatic involvements. Anti-P positivity and the titer of anti-P antibodies also fluctuate with clinical disease activity. SLE is nearly always diagnosed months to years after the onset of clinical symptoms and as we have previously shown, even longer after the onset of autoantibodies. Antibodies against ribosomal P frequently develops before clinical SLE diagnosis.