Carlo Massaroni was born in Anzio (Rome) in 1988.
He received the B.S. Degree in Biomedical Engineering from Università Campus Bio-Medico di Roma in December 2010. The Bachelor thesis title was "Perception Analysis of the form by the use of a numerical chessboard" and it was carried out in collaboration with the Bioinformatics Lab of the same University.
He received the M.S. Degree with highest honors in Biomedical Engineering from Università Campus Bio-Medico di Roma in December 2012. His graduation Thesis was entitled "Preliminary validation of a respiratory apparatus simulator for the assessment of metrological characteristics of a optoelectronic plethysmograph".
With his thesis he won two relevant National Price. On September he has been awarded the 2013 SIAMOC Dissertation Prize by the Italian Society of Clinical Movement Analysis (SIAMOC) in recognition of the outstanding quality of his undergraduate research. On October he has been awarded the 2013 GNB Dissertation Price by the Italian National Bioengineering Group (GNB) for outstanding contribute in the field of Rehabilitation and Prosthetics.
From April 2013 to January 2014 he worked as Biomechanical Researcher in the Unit of Rehabilitation at Scientific Institute for Research IRCCS Eugenio Medea of Conegliano (TV), as Scholarship Winner and Assignee for 2013.
He was PhD Student in Biomedical Engineering at Università Campus Bio-Medico di Roma from January 2014 to December 2016: he started working in the Laboratory of Measurements and Biomedical Instrumentation in January 2014, under the supervision of Prof. Sergio Silvestri.
In 2014 he has achieved the Professional Qualification as Industrial Engineer (Section A). He was Jury Aggregate Member as Topic expert in Biomedical Instrumentation during the State Examinations enabling the exercise of the profession of Engineer on behalf of Ordine degli Ingegneri of Rome. Section A - Industrial Section.
From February 2015 is Student Mentor for the International Biomechanics Society (ISB) in Instrumentation for Human Motion, Optoelectronic Systems and Optoelectronic Plethysmography.
On July 2015 he has been awarded the International Grant Travel Program Award by the International Society of Biomechanics (ISB) with the project "MoCBA - MOtion Capture for Breathing Assessment". MoCBA is an interdisciplinary project taking advantage of the research cooperation between the School of Sport & Exercise Sciences (SSES) at the University of Kent (Dr. Dickinson and Dr. Winter) and the School of Engineering, Research Unit of Measurement and Biomedical Instrumentation (UCBM) at the Campus Bio-Medico di Roma University. He is currently (until 22/04/2016) a Visiting PhD Student at University of Kent (Medway Campus, Kent, UK), where he is working in the Respiratory Clinic Unit.
On September 2017 he has been awarded the 2017 GNB Thesis Award by the Italian National Bioengineering Group (GNB) for outstanding contribute in the field of Biorobotics and Biomechanics.
2017 IEEE Sensor Council Travel Award (€ 1000) to attend the 2nd IEEE Sensors Summer
School on Optical Fibre Sensors at the University of Limerick, Ireland. 26th – 30th
June 2017 - Limerick, Ireland.
2016 European Society of Biomechanics (ESB) Travel Award (€ 400) to attend the 22nd
Congress of the European Society of Biomechanics (Respiratory Biomechanics
Session) - Lyon, France.
2015 International Society of Biomechanics (ISB) International Travel Grant (ITG2015) ($
2500) to fund the Research Project “MoCBA: Motion Capture for Breathing
Assessment” and to fund the foreign period of 6 months at University of Kent (UK).
Number of Publications: 43
Medical Image Analysis and
Processing Computer Simulations of
Nanoscale Systems Communications and
Mobile Computing Bioinformatics and
Computational Biology Computational Neuroscience and Vascular Flow Dynamics Biometrics
and Bio-robotics Computer Aided Diagnosis and Guided Surgerya
Medical Physics, Health
physics, Radiation detection, Technique of Radiation Therapy, Treatment
Planning in Radiation Therapy.
models in rats and mice; myocardial ischemia; myocardial infarction
Research Interest: Applied research specifically in tissue engineering, biomaterials and regenerative medicine. Currently focus is bionanomedicine, bionanocomposites and bionanotechnology
Research Interest: Electroporation, Electroloading,
Electrochemotherapy, Drug and Gene Delivery Systems, Molecular Imaging (EPR
imaging; optical imaging; multimodal imaging – MRI/EPR, MRI/optical), Cancer
Research Interest: Design, fabrication, and optical
properties of nanomaterials,- Biomedical applications (biosensing, bioimaging,
therapy) of nanomaterials ,- Surface-enhanced Raman scattering and related
Research Interest: His research focuses on computer
assisted surgery, including medical image analysis, surgical navigation,
surgery simulation, surgical robots, biomedical manufacturing, etc.
Research Interest: Designing and preparing new nanobiomaterials Characterizing
physical and chemical properties, biocompatibility and bioactivity of the
Research Interest: Cell Printing, Now, he is especially
interested in the novel cell printing method. With the different own designed
cell printers, the project about how to printing vessels and the organ at the
same time is underway. Organ on a chip, Now, he is especially interested in
designing new method to 3D printing microfluidic chips which can be used in cell
culture and the prototype of organ
Research Interest: His research interests concentrate on the
biosensors (e.g. living cell sensor, DNA sensor and protein sensor) and BioMEMS
Research Interest: His research interests concentrate on the
biosensors (e.g. living cell sensor, DNA sensor and protein sensor) and BioMEMS
Research Interest: Biofabrication, Selective laser sintering, Nanotechnology,
3D Printing Biomaterials, Tissue Engineering
Research Interest: Biomedical
instrumentation, Health monitoring
system, System modeling
and control, robotics
Research Interest: Non-point source, soil heavy metal, absorbing Material, and restoration
technology; Stochastic Optical Reconstruction Microscopy(STORM); Cell Imaging
and Structure; Propagation and Scattering of Light in Random and Complex Media;
Classical Coherence Theory; Laser Communications and LaserRadar Systems;
Near-field Scattering; Optical Imaging System
Medical Image Computing and
Analysis, Image registration, Reconstruction and Segmentation, Image
Computer Vision, Machine Vision, Pattern Recognition, Machine Learning, Motion
Registration, Optical Flow, Variational Method and Partial Differential
Numerical Method, Optimization, Image Guided Surgery, Matrix Computation,
Scientific Visualization, Cardiac Image Analysis, Brain Image Registration,
Brain Functional Mapping,
Image / signal processing,
Machine learning, Computer-aided clinical diagnosis and treatment selection
Optofluidics, Surface Wettability, Drug Delivery
Functional Brain Imaging, Pre-surgical
Brain Mapping, Biomedical signal and Image Processing, Neural Engineering
Numerical analysis finite
element method finite element analysis
engineering, computational simulations biomechanics heart heart valve
valve cardiovascular cardiovascular flow fluid-structure interaction
My research focuses on establishing the clinical applicability of two new diagnostic parameters (to detect coronary artery disease) using a set of bench-top experiments, computational analysis, pre-clinical and clinical trials in our research group. These proposed diagnostic parameters based on fluid dynamic principles, pressure drop coefficient and lesion flow coefficient, have the potential to make a real paradigm shift in clinical decision making related to cardiovascular intervention. As part of my research involving pre-clinical and clinical trials I had collaborated with clinicians and scientists at College of Medicine, University of Cincinnati and at Cincinnati Children's Hospital and Medical Center. Preliminary results from these studies have concluded the clinical applicability of these parameters to distinguish between stenoses of various severity.
More recently, at Weill Cornell Medical College, I am working on using 3D printing, machine learning and computational fluid dynamics to predict and prevent coronary artery disease from non-invasive CCTA imaging data.
Research Interest: 3D printing, Computational fluid dynamics, clinical radiology, non-invasive CCTA scanning, machine learning, interventional cardiology, applied mathematics, bio-heat transfer
List of Publications:
1. Al'Aref SJ, Anchouche K, Singh G, Slomka PJ, Kolli KK, Kumar A, Pandey M, Maliakal G, van Rosendael AR, Beecy AN, Berman DS, Leipsic J, Nieman K, Andreini D, Pontone G, Schoepf UJ, Shaw LJ, Chang HJ, Narula J, Bax JJ, Guan Y, Min JK. Clinical applications of machine learning in cardiovascular disease and its relevance to cardiac imaging. Eur Heart J. 2018. Epub 2018/07/31. doi: 10.1093/eurheartj/ehy404. PubMed PMID: 30060039. Available from: https://www.ncbi.nlm.nih.gov/pubmed/30060039.
2. Kolli KK, Min JK. Image-based computational fluid dynamic analysis for surgical planning of sequential grafts in coronary artery bypass grafting. Accepted as Contributed full paper for publication in Conference Proceedings for IEEE Engineering in Medicine and Biology Society Conference. 2018. [Accepted]
3. van Rosendael AR, Maliakal G, Kolli KK, Beecy A, Al'Aref SJ, Dwivedi A, Singh G, Panday M, Kumar A, Ma X, Achenbach S, Al-Mallah MH, Andreini D, Bax JJ, Berman DS, Budoff MJ, Cademartiri F, Callister TQ, Chang HJ, Chinnaiyan K, Chow BJW, Cury RC, DeLago A, Feuchtner G, Hadamitzky M, Hausleiter J, Kaufmann PA, Kim YJ, Leipsic JA, Maffei E, Marques H, Pontone G, Raff GL, Rubinshtein R, Shaw LJ, Villines TC, Gransar H, Lu Y, Jones EC, Pena JM, Lin FY, Min JK. Maximization of the usage of coronary CTA derived plaque information using a machine learning based algorithm to improve risk stratification; insights from the CONFIRM registry. J Cardiovasc Comput Tomogr. 2018;12(3):204-9. Epub 2018/05/14. doi: 10.1016/j.jcct.2018.04.011. PubMed PMID: 29753765. Available from: https://www.ncbi.nlm.nih.gov/pubmed/29753765.
4. Singh G, Al'Aref SJ, Van Assen M, Kim TS, van Rosendael A, Kolli KK, Dwivedi A, Maliakal G, Pandey M, Wang J, Do V, Gummalla M, De Cecco CN, Min JK. Machine learning in cardiac CT: Basic concepts and contemporary data. J Cardiovasc Comput Tomogr. 2018; 12(3):192-201. Epub 2018/05/15. doi: 10.1016/j.jcct.2018.04.010. PubMed PMID: 29754806. Available from: https://www.ncbi.nlm.nih.gov/pubmed/29754806.
5. Beecy AN, Chang Q, Anchouche K, Baskaran L, Elmore K, Kolli KK, Wang H, Al'Aref S, Pena JM, Knight-Greenfield A, Patel P, Sun P, Zhang T, Kamel H, Gupta A, Min JK. A Novel Deep Learning Approach for Automated Diagnosis of Acute Ischemic Infarction on Computed Tomography. JACC Cardiovascular imaging. 2018. Epub 2018/05/21. doi: 10.1016/j.jcmg.2018.03.012. PubMed PMID: 29778866. Available from: https://www.ncbi.nlm.nih.gov/pubmed/29778866.
6. Peelukhana SV, Banerjee R, van de Hoef TP, Kolli KK, Effat M, Helmy T, Leesar M, Kerr H, Piek JJ, Succop P, Back L, Arif I. Evaluation of lesion flow coefficient for the detection of coronary artery disease in patient groups from two academic medical centers. Cardiovascular Revascularization
Kranthi Kolli 6 10/05/2018Medicine. 2018;19(3 Pt B):348-54. Epub 2017/10/19. doi: 10.1016/j.carrev.2017.08.018. PubMed PMID: 29037762. Available from: https://www.ncbi.nlm.nih.gov/pubmed/29037762.
7. Han D, Starikov A, Xiong G, Hartaigh B, Granser H, Kolli KK, Lee J, Rizvi A, Baskaran L, Schulman-Marcus J, Lin FY, Min JK. Relationship between endothelial wall shear stress and high-risk atherosclerotic plaque characteristic for identification of coronary lesions that cause ischemia: A direct comparison to fractional flow reserve. Journal of the American Heart Association. 2016; 5(12). doi: 10.1161/JAHA.116.004186. PubmMed PMID: 27993831. Available from: https://www.ncbi.nlm.nih.gov/pubmed/27993831
8. Kolli KK, Min JK, Ha S, Soohoo H, Xiong G. Effect of Varying Hemodynamic and Vascular Conditions on Fractional Flow Reserve: An In Vitro Study. Journal of the American Heart Association. 2016;5(7). doi: 10.1161/JAHA.116.003634. PubMed PMID: 27364988. Available from: http://www.ncbi.nlm.nih.gov/pubmed/27364988.
9. Kolli KK, van de Hoef TP, Effat MA, Banerjee RK, Peelukhana SV, Succop P, Leesar MA, Imran A, Piek JJ, Helmy TA. Diagnostic cutoff for pressure drop coefficient in relation to fractional flow reserve and coronary flow reserve: A patient-level analysis. Catheterization and cardiovascular interventions : official journal of the Society for Cardiac Angiography & Interventions. 2016;87(2):273-82. doi: 10.1002/ccd.26063. PubMed PMID: 26424295. Available from: http://www.ncbi.nlm.nih.gov/pubmed/26424295.
10. Peelukhana SV, Effat M, Kolli KK, Arif I, Helmy T, Leesar M, Kerr H, Back LH, Banerjee R. Lesion flow coefficient: a combined anatomical and functional parameter for detection of coronary artery disease--a clinical study. The Journal of invasive cardiology. 2015;27(1):54-64. PubMed PMID: 25589702. Available from: http://www.ncbi.nlm.nih.gov/pubmed/25589702.
11. Peelukhana SV, Banerjee R, Kolli KK, Fernandez-Ulloa M, Arif I, Effat M, Helmy T, Kerr H. Benefit of ECG-gated rest and stress N-13 cardiac PET imaging for quantification of LVEF in ischemic patients. Nuclear medicine communications. 2015;36(10):986-98. doi: 10.1097/MNM.0000000000000352. PubMed PMID: 26225941. Available from: http://www.ncbi.nlm.nih.gov/pubmed/26225941.
12. Peelukhana SV, Kolli KK, Leesar MA, Effat MA, Helmy TA, Arif I, Schneeberger EW, Succop P, Banerjee RK. Effect of myocardial contractility on hemodynamic end points under concomitant microvascular disease in a porcine model. Heart and vessels. 2014;29(1):97-109. doi: 10.1007/s00380-013-0355-9. PubMed PMID: 23624760. Available from: http://www.ncbi.nlm.nih.gov/pubmed/23624760.
13. Peelukhana SV, Kerr H, Kolli KK, Fernandez-Ulloa M, Gerson M, Effat M, Arif I, Helmy T, Banerjee R. Benefit of cardiac N-13 PET CFR for combined anatomical and functional diagnosis of ischemic coronary artery disease: a pilot study. Annals of nuclear medicine. 2014;28(8):746-60. doi: 10.1007/s12149-014-0869-y. PubMed PMID: 24950752. Available from: http://www.ncbi.nlm.nih.gov/pubmed/24950752.
14. Kolli KK, Helmy TA, Peelukhana SV, Arif I, Leesar MA, Back LH, Banerjee RK, Effat MA. Functional diagnosis of coronary stenoses using pressure drop coefficient: a pilot study in humans. Catheterization and cardiovascular interventions : official journal of the Society for Cardiac Angiography & Interventions. 2014;83(3):377-85. doi: 10.1002/ccd.25085. PubMed PMID: 23785016. Available from: http://www.ncbi.nlm.nih.gov/pubmed/23785016.
15. Kolli KK, Effat MA, Peelukhana SV, Succop P, Back LH, Leesar MA, Helmy TA, Imran A, Banerjee RK. Hyperemia-free delineation of epicardial and microvascular impairments using a basal index. Annals of biomedical engineering. 2014;42(8):1681-90. doi: 10.1007/s10439-014-1020-x. PubMed PMID: 24806315. Available from: http://www.ncbi.nlm.nih.gov/pubmed/24806315.
Kranthi Kolli 7 10/05/2018
16. Kolli KK, Arif I, Peelukhana SV, Succop P, Back LH, Helmy TA, Leesar MA, Effat MA, Banerjee RK. Diagnostic performance of pressure drop coefficient in relation to fractional flow reserve and coronary flow reserve. The Journal of invasive cardiology. 2014;26(5):188-95. PubMed PMID: 24791716. Available from: http://www.ncbi.nlm.nih.gov/pubmed/24791716.
17. Kolli KK, Paul AK, Back LH, Effat MA, Banerjee RK. Optimization of balloon obstruction for simulating equivalent pressure drop in physiological stenoses. Biorheology. 2013;50(5-6):257-68. doi: 10.3233/BIR-130640. PubMed PMID: 24398608. Available from: http://www.ncbi.nlm.nih.gov/pubmed/24398608.
18. Peelukhana SV, Banerjee RK, Kolli KK, Effat MA, Helmy TA, Leesar MA, Schneeberger EW, Succop P, Gottliebson W, Irif A. Effect of heart rate on hemodynamic endpoints under concomitant microvascular disease in a porcine model. American journal of physiology Heart and circulatory physiology. 2012;302(8):H1563-73. doi: 10.1152/ajpheart.01042.2011. PubMed PMID: 22287585. Available from: http://www.ncbi.nlm.nih.gov/pubmed/22287585.
19. Kolli KK, Banerjee RK, Peelukhana SV, Effat MA, Leesar MA, Arif I, Schneeberger EW, Succop P, Gottliebson WM, Helmy TA. Effect of changes in contractility on pressure drop coefficient and fractional flow reserve in a porcine model. The Journal of invasive cardiology. 2012;24(1):6-12. PubMed PMID: 22210582. Available from: http://www.ncbi.nlm.nih.gov/pubmed/22210582.
20. Rajabi-Jaghargh E, Kolli KK, Back LH, Banerjee RK. Effect of guidewire on contribution of loss due to momentum change and viscous loss to the translesional pressure drop across coronary artery stenosis: an analytical approach. Biomedical engineering online. 2011;10:51. doi: 10.1186/1475-925X-10-51. PubMed PMID: 21658283; PMCID: 3141581. Available from: http://www.ncbi.nlm.nih.gov/pubmed/21658283.
21. Kolli KK, Banerjee RK, Peelukhana SV, Helmy TA, Leesar MA, Arif I, Schneeberger EW, Hand D, Succop P, Gottliebson WM, Effat MA. Influence of heart rate on fractional flow reserve, pressure drop coefficient, and lesion flow coefficient for epicardial coronary stenosis in a porcine model. American journal of physiology Heart and circulatory physiology. 2011;300(1):H382-7. doi: 10.1152/ajpheart.00412.2010. PubMed PMID: 20935151. Available from: http://www.ncbi.nlm.nih.gov/pubmed/20935151.
Time-domain optical brain
imaging, functional near-infrared ectroscopy,
fluorescence measurements, analyses of
an optical properties of the media, development of optical imaging systems, brain
optics, clinical applications
Tissue & organ repair and regeneration, Tissue engineering, Wound healing,
Clinical medicine, dimensional tissue equivalent models, Novel wound and scar
remediation therapies, Chinese herbal medicine, Growth factor/extracellular
matrix protein complexes, Pharmaceutical and drugs
Surgical planning and
simulation in esthetical facial surgeryTelemedicine o Biomedical engineering
Development of new
lanthanide complexes for MRI and optical imaging: synthesis, purification
(HPLC, flash chromatography) and physicochemical characterization (NMR
spectroscopy, mass spectrometry, luminescence, UV-visible, FTIR).
Acetylcholinesterase reactivators as treatment of nerve agent and pesticide
intoxications; Acetylcholinesterase inhibitors in Alzheimer´s disease and Myasthenia
Gravis treatment; Synthesis of the detergents as disinfectants, decontamination
means and environment for micellar catalysis; Drug design and Development;
Chemical warfare agents; Biological warfare agents; Pharmaceutical Industry;
Project Management; Scientific Management; Technology Transfer;
Commercialization; Fund Raising; Cloud computing; Parallel computing; medical
devices development; toxins.
Knowledge Management, Cost Benefit Analysis Method – application in company and
in medical device market development, Healthcare, efectivness of medical
mechanical properties and corrosion behaviour of biomaterials Mechanical
surface treatments (shot peening and ball-burnishing) of metals and alloys. Coatings
prior and after mechanical surface treatments. Evaluating the effects of Severe
Plastic Deformations (SPD) on the materials. Characteristics Evaluation of
surface layer properties (surface roughness, micro-hardness and residual stresses)
of metals and alloys. Fatigue, corrosion fatigue and stress corrosion cracking
of metals and alloys. Corrosion monitoring by electrochemical techniques. Analytical
assessments by SEM and EDX. Analytical assessments by spectrophotometer and
Connective Tissue, Drugs
Action, Microscopy, Protein Modeling, Bone Structure, Trace/major Elements,
Image Processing, Electromagnetism, Spectroscopy, Biometrics, Human Networks
Since 2008 Paola Saccomandi
has been involved in research activities in the fields of biomedical
instrumentations and measurements applied to medicine. From 2008 to 2011 she
worked under the supervisio of Prof. Sergio Silvestri and Dr. Emiliano Schena
in the fied of mechanical ventilation. In particular she worked on development
of optoelectronic and fiber optic-based flow sensors, as well as on ventilatory
gases humidification. In 2011 she started her main research activity in the
field of laser ablation of cancer, with particular regard to pancreatic tumor.
She developed a mathematic model aiming to predic effects of laser light o
pancreas, in terms of ablated volume and temperature distribution. Since 2012
she has been investigating the field of invasive (fiber optic sensors,
thermocouples) and non-ivasive (based on diagnostic images) thermometry for
temperature monitoring during laser ablation of tumour. In this activity she is
actively collaborating with the team of research of the Institute for
Diagnostic and Interventional Radiology of J. W. Goethe Universität Frankfurt
am Main, headed by Prof. Thomas J. Vogl. Since 2013 she has been working in the
fied of optical characterization of biological tissue, aiming to estimate
optical properties of pancreas. She is collaborating with the team of
Biophysics Institute J. W. Goethe Universität Frankfurt am Main, headed by
Prof. Werner Mäntele. In the framework of laser ablation for the removal of
pancreatic cancer, she has participated at the study approved by UCBM Ethics
Committee -ComEt UCBM-, Prot. N. 4011/2011. The study aims to treat with laser
ablation patients with inoperable pancreatic cancer. Since 2014 she is also
involved in the design and development of tactile sensors for robotic
Scaffold Design For Tissue
Engineering, Composites hydrogels For Bone Repair, Electrospinning and
Electrospraying technology, Scanning and Trasmission Electron Microscopy, Image
Analysis For Porosity Measurements, Chemical And Physical Properties
Characterization Of Composite And/Or Polymeric Substrates, Injectable Composite
Cements For Bone Cavity Filling.
Research Interest: Synthesis and characterization of inorganic and hybrid organic-inorganic nanomaterials, calcium phosphates, biomaterials, drug delivery, biomineralization and nanomedicine.
Breathing assessment and
analysis,Human movement analysis;Bio-mechanical analysis, Opto-electronic
Plethysmography, Gait analysis; Energy consumption, Devices and procedures for
optoelectronic/motion analysis systems calibration and metrological assessment;
Design and validation of devices for breathing assessment and/or simulation
Stem cells and Regenerative
Medicine, Tissue Regeneration, Tissue Engineering Stem Cells Biology and
Differentiation, Stem cells biomaterials interaction, Mechanotransduction
fatigue, motor units, innervation zone, anal sphincter, MMG, biofeedback,
cervical kinematics, helical axis
Biophotonics and Biosensors
His current research
interests include non-linear analysis of biomedical time series, specially the
analysis of electroencephalographic and magnetoencephalographic recordings to
help physicians in the diagnosis of brain disorders
Cell and tissue engineering
Shape memory materials and
composites (bulk and thin film), Laser annealing and micro fabrication, Thin
films (characterization and materials/devices) Micro/nano mechanics, nano
patterning and indentation technology, Surface and optical properties of smart
materials. Advanced and novel materials.
Micro assembly and packaging, active
disassembly. Deployable and bi-stable structures. Materials selection Flight
mechanism in inserts Micro biomedical devices
Bone and cartilage disease
modeling, drug screening and gene correctionDirect differentiation of
pluripotent stem cells toward cartilage and boneBone reprogramming of fibroblasts
using defined factors Gene therapy for cartilage regeneration and repair in
regenerative medicine Signaling pathway of genes regulating cartilage
differentiation of human mesenchymal stem cells.Molecular basis of mesenchymal
stem cells maintaining their stemness. Stem cell biology and technique
Protein engineering and
protein chemistry, Nanobiotechnology and Protein nanocage, Biomaterials and
Biomedical engineering, Industrial microbiology
Research Interest: Basic experiment skills in molecular and cell biology, immunology and zoology as follows: Molecular biology:Regular skills of molecular biology;plasmid construction, including shRNA and overexpression, GFP fusion protein, nucleic acid extraction, purification. Biochemistry: protein expression analysis, including western blotting, immunoprecipitation and quantitative real-time RT-PCR; E.coli expressionsystem, soluble and insoluble protein purification. Cell biology: Mammalian cell culture; plasmid transfection; development of stable transfected cell lines; immunohistochemistry; immunocytochemistry. Others: Basic animal, especially mouse and rat handling skills;drosophila culture; familiar with ordinary operation of laser scanning confocal microscopy (Leica,Zeiss,Olympus), fluorescence microscopy, fluorescence spectrometer;familiar with ordinary statistic and bioinformatical softwares.
Development, Cellular and Tissue Engineering Drug and Gene Delivery, Molecular
Diagnostics Photobioreactor Design
Cardiovascular Research Foundation, New York, USA
Archives of Clinical Hypertension
Leeds Beckett University, UK
Archives of Community Medicine and Public Health
Peter J Catalano
Tufts University, USA
Archives of Otolaryngology and Rhinology
Domenico Antonio Restivo
Nuovo Garibaldi Hospital, Italy
Journal of Neurology, Neurological Science and Disorders
Taipei Medical University, Taiwan
Archives of Nursing Practice and Care
Jose Manuel Ramia-Angel
Guadalajara University Hospital , Spain
Journal of Surgery and Surgical Research
Democritus University of Thrace , Greece
International Journal of Vascular Surgery and Medicine
Sassari University, Italy
Archive of Urological Research
University of Nantes, France
Archives of Depression and Anxiety
Catholic University of Rome, Italy
Open Journal of Orthopedics and Rheumatology