Thomas F. George
University of Missouri - St. Louis, USA
Research Interest: Analytical Probe and Protein Biotechnology (a branch of chemical biology) Based on physicochemical principle for interactions of proteins with their ligands, chemometrics is utilized to mine information associated with the forces of their interactions, the strength for their bioaffinity interactions (affinities), the quantities of counterpart biomolecules, which facilitates the development of methods and probes for in vitro diagnostics and drug screening, protein ligands and protein mutants of biological significance. In detail, our research interest covers the following topics:
(1) New methods and probes for in vitro diagnostics and drug screening: to enhance analytical efficiency and precision, probes are designed based on mechanistic ways of bioaffinity interactions and new process to mine information are developed; such new methods and probes are applied to simultaneous assays of multiple components in single channel, especially for simultaneous immunoassays of multiple components in single channel, the screening of ligand mixtures, the screening of enzyme mutants, homogenous bioaffinity assay with tryptophan residues as resonance-energy-transfer donors, and linear response by competitive bioaffinity assay;
(2) With PDE and GST as examples, methods are developed to rational and combinatorial design of isozyme-selective inhibitors, pro-inhibitors and soft inhibitors, the screening of such inhibitors, and the potential as therapeutic agents;
(3) With uricase, arylsulfatase and alkaline phosphatase as examples, rational design and directed evolution of enzymes are studied, together with high-throughput screening and evaluation of their mutants, chemical modification of proteins; rational design of fused proteins as therapeutic agents;
(4) Affinity-labeling and site-specific immobilization of protein targets, the cross-linking/conjugation of enzymes and antibodies.
Research Interest: Spectrophotometry, Spectrofluorimetry, High performance liquid chromatography, Atomic absorption spectrophotometry
Research Interest: Chemical sensors,Flow injection analysis,Drug analysis,Food safety analysis,Chemometrics,Robust statistics, Correspondence analysis, Three-way data analysis, Multivariate calibration, Second-order calibration, Second-order standard addition method，Multilinear decomposition，Multiway calibration,Novel applications of multi-way data analysis and multiway calibration methodologies to analytical, environmental, biological, drug, medical, food and life sciences,Quantitative analysis of proteins, metabonomics,Multi-way data analysis in automation and control systems
Research Interest: Screening small molecules that inhibit signaling pathways in cancer; Studying DNA damage and repair, cell cycle control, apoptosis and autophagy in cancer; Studying roles of protein modifications such as phosphorylation and ubiquitination in cancer; Investigating the roles of DNA polymerase epsilon (Polε) in telomere length maintenance
Research Interest: Metallic nanomaterials; Gold nanoparticles; Elemental mass spectrometry; Analytical chemistry; Atomic spectrometry
Research Interest: Fluorescent Sensors for HTM,On line coupled GC/MS or LC-MS/MS analysis of POPs
Research Interest: Tuberculosis ,Infectious Diseases, M.tuberculosis, Proteomics and related Bioinformatics, Electrophoresis, SDS-PAGE, 2D gel electrophoresis, Cloning, Expression, Purification, Molecular Modelling, Docking, Post translational modifications, Pupylation, Protein extraction and method devlopment for 2D, Mass Spectrometry, MALDI-TOF/MS and MS/MS etc
Research Interest: Arthritis, Stress, Neuropharmacology, Diabetes
Research Interest: Fluorescent chemosensors, nanomaterials (nanoparticles, nanorods, nanofibers, nanocomposites), heavy-metal(Ag+, Pb2+, Hg2+) ion sorbents, and ion-selective electrodes of intrinsically electrically conducting/conjugated polymers from aniline, pyrrole, thiophene, pyrene, triphenylene, and their derivatives; Synthesis of functional polymers, polymer chemistry; Liquid-crystalline aromatic polyesters and cholesteric cellulose derivatives; Water treatment and purification, Separation membrane science
Research Interest: Synthesis of anostructured materials (e.g.Gold nanoparticles, Carbon Nanotube, QDs), Assembly of biomimetic nanomaterial and photoelectric chemical analysis, Development of protein and cell based electrochemical biosensors for medical diagnostics, Fundamental Understanding of DNAs, Antibodies, DNAzymes and Their Applications as Sensing and Imaging Agents in Environmental Monitoring and Medical Diagnostics
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Published On: November 05, 2019 | Pages: 065 - 071Abstract View Full Article View
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