Dr. Monther AlAlwan is a scientist at the Stem Cell and Tissue Re-engineering Program (SCTRP), King Faisal Specialist Hospital and Research Centre, as well as an Associate professor at Alfaisal University, Riyadh Saudi Arabia. He holds M.Sc. and Ph.D. in Immunology from Dalhousie University (Halifax, Canada). Dr. AlAlwan conducted a 3-years postdoctoral fellowship studying signaling in immune cells at the University of Manitoba, Canada. During his graduate studies and postdoctoral fellowship, Dr. AlAlwan made substantial contribution in the immunology field, particularly his groundbreaking discovery that highlighted the significance of the dendritic cells in the immunological synapse. After joining the SCTRP in 2007, he shifted gear to study cancer, where he identified novel mechanisms that regulate cancer metastasis and chemoresistance. Currently, he is actively involved in dissecting the molecular pathways that regulate the function of cancer stem cells and how this related to chemoresistance and metastasis. Dr. AlAlwan is an author in 20 peer-reviewed publications, has delivered several invited lectures and is a regular reviewer for various international journals.
2006 – 2009
M.S. in Microbiological and Biochemical Pharmacy
Shanghai Jiaotong University, Shanghai, China
2001 - 2005
B.S. in Biotechnology (Fermentation Engineering)
Shanghai Normal University, Shanghai, China
Stem Cell Research and Therapy
Gynecological Disease Research
Autoimmune Disease Research
RESEARCH INTERESTS AND SKILLS
My scientific interests are to understand how genetic and molecular programs can yield the complex biological behaviors. As a medical research scientist, synthetic biologist and bioengineer, I am passionate about the applications of diverse biotechnological tools to understand genomics and explore the mechanism of genomic control as well as incidence of diseases, which allow comprehensive understanding, manipulating, and engineering of sophisticated biological systems. I aim to understand cellular or organismal behaviors in both space and time thus benefit the biomedical research, translational studies and cell therapy.
I am skilled in cell culture (primary/linage) and cellularity detections (CCK8, FACS etc); molecular cloning and detection (plasmid construction, RT-PCR, western blot etc); protein [removed]prokaryotic expression system) and purification (ionexchange and affinity chromatography); immunohistochemistry and immunocytochemistry detection; and animal model construction.
Research Grant Details:
National Natural Science Foundation of China (Grant No. 81502233)
Existing evidence indicates that PPI (protein-protein interaction) contributes to the tumorigenesis of endometrial carcinoma, but the mechanisms involved in it remain largely unknown. In our previous study, we found that ATF3 participating in the development of various different kinds of cancer, expressed higher in the epithelium of normal endometrial tissue than that detected in pathnological tissue, and interacted with JunB which expressing higher in the epithelium of endometrial cancer tissue than that detected in normal endometrial tissue, via yeast-two-hybrid assay. Additionally, over-expression of ATF3 led to the inhibition of cell proliferation, migration and invasion, with the down-regulation of JunB and inflammatory factor. Therefore, we put forward a hypothesis that ATF3 might inhibit the expression of inflammatory genes and the development of endometrial cancer by binding with JunB andmitigating with it. This study was designed to utilize the mRNA expression microarray analysis, CRISPR/Cas9 and orthotopic endometrial carcinoma model in nude mice combined with molecular biological techniques to explore the cell signal transductions of ATF3 and JunB in EC, demonstrate the mechanism of development of EC, and provide new therapeutic targets for it, which contributes greatly to the patients suffering from endometrial cancer.
1． Wang Fangyuan(#), Qi Stanley Lei(*). Applications of CRISPR Genome Engineering in Cell Biology. Trends in Cell Biology. 2016 Nov;26(11):875-888.
2． Wang Fangyuan(#), Zhao Dehua, Qi Lei Stanley(*). Application of genome engineering in medical synthetic biology. Sheng Wu Gong Cheng Xue Bao. 2017 Mar 25;33(3):422-435.
3． Wang Fangyuan(#), Gao Jin, Malisani Alyssa, Xi Xiaowei, Han Wei, Wan Xiaoping (*), Mouse Resistin (mRetn): cloning, expression and purification in Escherichia coli and the potential regulative effects on murine bone marrow hematopoiesis. BMC Biotechnol, 2015 Nov 16;15(1):105.
4． Wang Fangyuan(#), Wang Li, Yao Xiaofen, Lai Dongmei(*). Human amniotic epithelial cells can differentiate to granulosa cells and restore folliculogenesis in a mouse model of chemotherapy-induced premature ovarian failure. Stem Cell Res Ther. 2013 Oct 14;4(5):124-134.
5． Liu Yanxia(#), Yu Chen(#), Daley Timothy Patrick(#), Wang Fangyuan, Cao William S., Bhate Salil, Lin Xueqiu, Still II Chris, Liu Honglei, Zhao Dehua, Wang Haifeng, Xie Xinmin S., Ding Sheng, Wong Wing Hung, Wernig Marius, Qi Lei Stanley. CRISPR Activation Screens Systematically Identify Factors that Drive Neuronal Fate and Reprogramming. Cell Stem Cell. 2018 Nov 1;23(5):758-771.
6． Yan Qin(#), Wang Fangyuan, Miao Yi, Wu Xiaomei, Bai Mingzhu, Xi Xiaowei, Feng Youji(*). Sex-determining region Y-box3 (SOX3) functions as an oncogene in promoting epithelial ovarian cancer by targeting Src kinase. Tumour Biol. 2016 Sep;37(9):12263-12271.
7． Lai Dongmei(#)(*), Wang Fangyuan, Yao Xiaofen, Zhang Qiuwan, Wu Xiaoxing, Xiang Charlie(*). Human endometrial mesenchymal stem cells restore ovarian function through improving the renewal of germline stem cells in a mouse model of premature ovarian failure. J Transl Med. 2015 May 12;13:155.
8． Lai Dongmei(#)(*), Wang Fangyuan, Dong Zhangli, Zhang Qiuwan. Skin-derived mesenchymal stem cells help restore function to ovaries in a premature ovarian failure mouse model. PLoS One. 2014 May 30;9(5):e98749.
9． Lai Dongmei(#)(*), Wang Fangyuan, Chen Yifei, Wang Li, Wang Yanlin, Cheng Weiwei. Human amniotic fluid stem cells have a potential to recover ovarian function in mice with chemotherapy-induced sterility. BMC Dev Biol. 2013 Sep 4;13(1):34.
10． Lai Dongmei(#)(*), Wang Fangyuan, Chen Yifei, Wang Chunhui, Liu Sha, Lu Bufeng, Ge Xuerui, Guo Lihe. Human ovarian cancer stem-like cells can be efficiently killed by γδ T lymphocytes. Cancer Immunol Immunother. 2012 Jul;61(7):979-89.
11． Che Qi(#), Liu Binya, Wang Fangyuan, He Yinyan, Lu Wen, Liao Yun, Gu Wei, Wan Xiaoping(*). Interleukin 6 promotes endometrial cancer growth through an autocrine feedback loop involving ERK-NF-kappaB signaling pathway. Biochem Biophys Res Commun, 2014, 446(1):167-172.
12． Lai Dongmei(#)(*), Ma Li, Wang Fangyuan. Fibroblast activation protein regulates tumor-associated fibroblasts and epithelial ovarian cancer cells. Int J Oncol. 2012 Aug;41(2):541-50.
13． Lai Dongmei(#)(*), Chen Yifei, Wang Fangyuan, Jiang Lizhen, Wei Chunsheng. LKB1 Controls the Pluripotent State of Human Embryonic Stem Cells. Cellular Reprogramming. April 2012, 14(2): 164-170.
14． Chen Zheng(#), Che Qi, He Xiaoying, Wang Fangyuan, Wang Huihui, Zhu Minjiao, Sun Jing(*), Wan Xiaoping(*). Stem cell protein Piwil1 endowed endometrial cancer cells with stem-like properties via inducing epithelial-mesenchymal transition. BMC Cancer, 2015 Oct 27;15:811.
15． Chen Zheng(#), Che Qi, Jiang Feizhou, Wang Huihui, Wang Fangyuan, Liao Yun, Wan Xiaoping(*). Piwil1 causes epigenetic alteration of PTEN gene via upregulation of DNA methyltransferase in type I endometrial cancer. Biochem Biophys Res Commun, 2015 Aug 7;463(4):876-80.
16． Liao Yun(#), He Xiaoying, Qiu Haifeng, Che Qi, Wang Fangyuan, Lu Wen, Chen Zheng, Qiu Meiting, Wang Jingyun, Wang Huihui, Wan Xiaoping(*). Suppression of the epithelial-mesenchymal transition by SHARP1 is linked to the NOTCH1 signaling pathway in metastasis of endometrial cancer. BMC Cancer. 2014, 14：487。
17． Huang Haili(#), Wang Yajing, Zhang Qingyu, Liu Bin, Wang Fangyuan, Li Jingjing, Zhu Runzhi(*). Hepatoprotective effects of baicalein against CCl4-induced acute liver injury in mice. World J Gastroenterol 2012 Dec 7;18(45):6605-6613.
18． Wang Huihui(#), Bao Wei, Jiang Feizhou, Che Qi, Chen Zheng, Wang Fangyuan, Tong Huan, Dai Chenyun, He Xiaoying, Liao Yun, Liu Binya, Sun Jing, Wan Xiaoping(*). Mutant p53 (p53-R248Q) functions as an oncogene in promoting endometrial cancer by up-regulating REGgamma. Cancer Lett. 2015 May 1;360(2):269-79.
19． Xia Peng(#), Gao Jin, Guan Wen, Li Jingjing, Yu Xiaolan, Wang Fangyuan, He Honglin, Deng Qing, Zhou Liang, Yuan Yunsheng, Han Wei(*), Yu Yan(*). Production of bioactive recombinant rat soluble receptor for advanced glycation end products (rrsRAGE) in Pichia pastoris. Protein Expr Purif, 2017 Oct;138:81-87.
20． Zhu Minjiao(#), Che Qi, Liao Yun, Wang Huihui, Wang Jingyun, Chen Zheng, Wang Fangyuan, Dai Chenyun, Wan Xiaoping(*). Oncostatin M activates STAT3 to promote endometrial cancer invasion and angiogenesis. Oncol Rep. 2015 Jul;34(1):129-38.
21． Che Qi(#), Liu Binya, Liao Yun, Zhang Huijuan, Yang Tingting, He Yinyan, Xia Yuhong, Lu Wen, He Xiaoying, Chen Zheng, Wang Fangyuan, Wan Xiaoping(*). Activation of a positive feedback loop involving IL-6 and aromatase promotes intratumoral 17beta-estradiol biosynthesis in endometrial carcinoma microenvironment. Int J Cancer. 2014 Jul 15;135(2):282-94.
22． Liu Yuan(#), Murray-Stewart T, Casero RA Jr, Kagiampakis I, Jin L, Zhang J, Wang H, Che Qi, Tong Huan, Ke Jieqi, Jiang Feizhou, Wang Fangyuan, Wan Xiaoping(*). Targeting hexokinase 2 inhibition promotes radiosensitization in HPV16 E7-induced cervical cancer and suppresses tumor growth. Int J Oncol. 2017 Jun;50(6):2011-2023.
23． Ke Jieqi(#), Yang Yixia(#), Che Qi, Jiang Feizhou, Wang Huihui, Chen Zheng, Zhu Minjiao, Tong Huan, Zhang Huilin, Yan Xiaofang, Wang X, Wang Fangyuan, Liu Yuan, Dai Chenyun, Wan Xiaoping(*). Prostaglandin E2 (PGE2) promotes proliferation and invasion by enhancing SUMO-1 activity via EP4 receptor in endometrial cancer. Tumour Biol. 2016 Sep;37(9):12203-12211.
24． Jiang Feizhou(#), He Yinyan, Wang Huihui, Zhang Huilin, Zhang Jian, Yan Xiaofang, Wang Xiaojun, Che Qi, Ke Jieqi, Chen Zheng, Tong Huan, Zhang Yongli, Wang Fangyuan, Li Yiran, Wan Xiaoping(*). Mutant p53 induces EZH2 expression and promotes epithelial-mesenchymal transition by disrupting p68-Drosha complex assembly and attenuating miR-26a processing. Oncotarget, 2015 Dec 29;6(42):44660-74.
(#) First Author, (*) Correspondence Author.
Two Major Projects are Currently Underway in the Laboratory.
A Cell Reprogramming Technique Has Been Used to Convert Heterogeneous Malignant Breast Cancer Cells into Induced Pluripotent Stem Cells Using Sox2/Oct4/Nanog Proteins. Dr. Wu’s Laboratory is Clarifying these Induced Pluripotent Stem Cells for their Differentiation Potential and Oncogenic Properties, and Try to Develop a Novel Cell Converting Therapy for Malignant Breast Cancer Treatment.
Metastasis, the Spread of Cancer Cells from the Primary Tumor To Distant Organs, Is The Most Dreadful Development Of Breast Cancer, As Well As Other Neoplastic Diseases. Although Metastasis Contributes To Over 90% Of Human Cancer Mortality, The Molecular Mechanism Of This Process Remains Largely Unknown. Dr. Wu’s Laboratory Is In The Process Of Identifying Molecular Signatures Involved In Breast Cancer Metastasis Using Integrative Genetic, Epigenetic And Proteomic Approach, As Well As Animal Models And Clinical Specimens.
Research Interest: Cell Reprogramming and Induced Pluripotent Stem
Cells, Stem Cells and Neural Tissue Engineering, Mammalian Inner Ear
Development and Hair Cell Regeneration.
Research Interest: Therapeutic Application of ES and Ips Cells.
Research Interest: Neuropsychopharmacology of Lipid Transmitters (CTS643,
Andalusian Ministry of Economy, Innovation, Science and Employment): The Group is
Formed in the University Regional Hospital of Malaga, in Collaboration with the
University of Malaga, and Integrated Into the Biomedical Research Institute of Malaga
(IBIMA). Our Interest Focuses on the Role that Regulatory Lipid Molecules,
Mainly Lysophosphatidic Acid, Exerted on the Central Nervous System and Related
Mainly to the Neural Genesis (Neurogenesis / Gliogenesis) and its Effect on
Behavioral Level. It Also Examines the Role of Lysophosphatidic Acid in the
Dynamic Processes that Regulate Neuroinflammation and Demyelination in Multiple
Sclerosis, Studying Animal Models and Samples from Patients with Multiple
Sclerosis, As Well As Mesenchymal Cell Therapy for the Disease.
Research Interest: ES/Ips Cells; Skeletal Muscle Regeneration; Gene and
Cell Therapy; Genome Editing; Gene Targeting.
Research Interest: Regenerative Biology (Marine Invertebrates and
Mammals), Stem Cell Biology, Developmental Biology.
Research Interest: Neurological Disability.
Research Interest: In his Consulting Experience, Devyn Led a Wide
Range of Projects, Across Multiple Therapeutic Areas and a Host of Technology
Platforms, Including Basic R&D Tools, Regenerative Medicine, Gene Therapy and
Research Interest: Cardiology, Toxicology, Epigenetics, Stem Cell
Differentiation, and Bioinformatics.
Research Interest: Cardiac Therapy, Cardiovascular Disease,
Autologous, Tissue-Engineering, Multipotent Stem Cells, Cardiovascular
Structures, Regenerative Medicine.
Research Interest: Cancer Stem Cell, Anticancer Agent, Metastasis, Cell
Survival, Cell Migration, Apoptosis, Molecular and Cell Signaling, Transcription
Factors, Xenograft Model, Stem Cell Based Therapy.
Research Interest: Leptin Receptors (Lepr) are Expressed by Various
Types of Stem Cells Including Mesenchymal Stem Cells, Hematopoietic Stem Cells,
Embryonic Stem Cells and Induced Pluripotent Stem Cells. Leptin/Lepr Signaling
is also a Central Regulator of Metabolism.
Research Interest: Embryonic Stem Cells, Pluripotency, Induced Pluripotent
Cells, Differentiation, Endoderm, Beta Cell, Diabetes, Obesity, Adipose Tissue,
Brown Fat, Endothelium, Oxidative Stress, Nitric Oxide, Peroxynitrite, Superoxide,
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